ArticleRNA (New York, N.Y.)2009
Novel application of Phi29 DNA polymerase: RNA detection and analysis in vitro and in situ by target RNA-primed RCA.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Reconstituting transcription-translation-coupled DNA replication within complex in vitro biological systems.Nature communications · 2025Article
- Visualizing the transcription and replication of influenza A viral RNAs in cells by multiple direct RNA padlock probing and in situ sequencing (mudRapp-seq).Nucleic acids research · 2025Article
- An evaluation of nucleic acid-based molecular methods for the detection of plant viruses: a systematic review.Virusdisease · 2024Review
- Review
- ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situ.Nature methods · 2022Article
- A Combinational Approach for More Efficient miRNA Biosensing.Current genomics · 2022Review
- Padlock probe-based rolling circle amplification lateral flow assay for point-of-need nucleic acid detection.The Analyst · 2021Article
- In vitro self-replication and multicistronic expression of large synthetic genomes.Nature communications · 2020Article
- Chimeric padlock and iLock probes for increased efficiency of targeted RNA detection.RNA (New York, N.Y.) · 2019Article
- ClampFISH detects individual nucleic acid molecules using click chemistry-based amplification.Nature biotechnology · 2018Article
- Research Progress on Rolling Circle Amplification (RCA)-Based Biomedical Sensing.Pharmaceuticals (Basel, Switzerland) · 2018Review
- Limited reverse transcriptase activity of phi29 DNA polymerase.Nucleic acids research · 2018Article
- Determination of RNase H activity via real-time monitoring of target-triggered rolling circle amplification.Mikrochimica acta · 2017Article
- Dual functional Phi29 DNA polymerase-triggered exponential rolling circle amplification for sequence-specific detection of target DNA embedded in long-stranded genomic DNA.Scientific reports · 2017Article
- Template-dependent multiple displacement amplification for profiling human circulating RNA.BioTechniques · 2017Article
- Simultaneous Single-CellJournal of virology · 2017Article
- Highly specific imaging of mRNA in single cells by target RNA-initiated rolling circle amplification.Chemical science · 2017Article
- Efficient in situ detection of mRNAs using the Chlorella virus DNA ligase for padlock probe ligation.RNA (New York, N.Y.) · 2017Article
- Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues.Nature protocols · 2015Article
- A novel ultrasensitive ECL sensor for DNA detection based on nicking endonuclease-assisted target recycling amplification, rolling circle amplification and hemin/G-quadruplex.Sensors (Basel, Switzerland) · 2015Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a novel Phi29 DNA polymerase application in RCA-based target RNA detection and analysis. The 3'-->5' RNase activity of Phi29 DNA polymerase converts target RNA into a primer and the polymerase uses this newly generated primer for RCA initiation. Therefore, using target RNA-primed RCA, padlock probes may be targeted to inner RNA sequences and their peculiarities can be analyzed directly. We demonstrate that the exoribonucleolytic activity of Phi29 DNA polymerase can be successfully applied in vitro and in situ. These findings expand the potential for detection and analysis of RNA sequences distanced from 3'-end.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.