ArticleJournal of virology2009
Mitochondrion-enriched anionic phospholipids facilitate flock house virus RNA polymerase membrane association.
Article in Journal of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 26 citations in OpenAlex.
- Alteration of mitochondrial function in arthropods during arboviruses infection: a review of the literature.Frontiers in physiology · 2025Review
- Review
- RNA virus replication depends on enrichment of phosphatidylethanolamine at replication sites in subcellular membranes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- The RNA binding of protein A from Wuhan nodavirus is mediated by mitochondrial membrane lipids.Virology · 2014Article
- Two membrane-associated regions within the Nodamura virus RNA-dependent RNA polymerase are critical for both mitochondrial localization and RNA replication.Journal of virology · 2014Article
- Viruses and antiviral immunity in Drosophila.Developmental and comparative immunology · 2014Review
- The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids.PloS one · 2014Article
- Flock house virus RNA polymerase initiates RNA synthesis de novo and possesses a terminal nucleotidyl transferase activity.PloS one · 2014Article
- Characterization of a nodavirus replicase revealed a de novo initiation mechanism of RNA synthesis and terminal nucleotidyltransferase activity.The Journal of biological chemistry · 2013Article
- Architecture and biogenesis of plus-strand RNA virus replication factories.World journal of virology · 2013Review
- Neurotropic arboviruses induce interferon regulatory factor 3-mediated neuronal responses that are cytoprotective, interferon independent, and inhibited by Western equine encephalitis virus capsid.Journal of virology · 2013Article
- The dependence of viral RNA replication on co-opted host factors.Nature reviews. Microbiology · 2011Review
- Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virus.Journal of virology · 2011Article
- Implications for lipids during replication of enveloped viruses.Chemistry and physics of lipids · 2010Review
- Role of cellular lipids in positive-sense RNA virus replication complex assembly and function.Viruses · 2010Article
- Article
- A targeted analysis of cellular chaperones reveals contrasting roles for heat shock protein 70 in flock house virus RNA replication.Journal of virology · 2010Article
- The rubella virus capsid protein inhibits mitochondrial import.Journal of virology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
One characteristic of all positive-strand RNA viruses is the necessity to assemble viral RNA replication complexes on host intracellular membranes, a process whose molecular details are poorly understood. To study viral replication complex assembly we use the established model system of Flock House virus (FHV), which assembles its replication complexes on the mitochondrial outer membrane. The FHV RNA-dependent RNA polymerase, protein A, is the only viral protein necessary for genome replication in the budding yeast Saccharomyces cerevisiae. To examine the host components involved in protein A-membrane interactions, an initial step of FHV RNA replication complex assembly, we established an in vitro protein A membrane association assay. Protein A translated in vitro rapidly and specifically associated with mitochondria isolated from yeast, insect, and mammalian cells. This process was temperature dependent but independent of protease-sensitive mitochondrial outer membrane components or the host mitochondrial import machinery. Furthermore, lipid-binding studies revealed that protein A preferentially bound to specific anionic phospholipids, in particular the mitochondrion-specific phospholipid cardiolipin. These studies implicate membrane phospholipids as important host determinants for FHV RNA polymerase membrane association and provide evidence for the involvement of host phospholipids in positive-strand RNA virus membrane-specific targeting.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.