ArticleProceedings of the National Academy of Sciences of the United States of America1991
In vivo catalysis of a metabolically essential reaction by an antibody.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 81 citations in OpenAlex.
- Exploring the "misfolding problem" by systematic discovery and analysis of functional-but-degraded proteins.Molecular biology of the cell · 2023Article
- Apoplastic and cytosolic expression of full-size antibodies and antibody fragments in Nicotiana tabacum.Transgenic research · 1999Article
- Bringing biological solutions to chemical problems.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- In vivo versus in vitro screening or selection for catalytic activity in enzymes and abzymes.Molecular biotechnology · 1997Review
- On the failure of de novo-designed peptides as biocatalysts.Proceedings of the National Academy of Sciences of the United States of America · 1996Review
- Expression studies of catalytic antibodies.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Selection of catalytic antibodies for a biosynthetic reaction from a combinatorial cDNA library by complementation of an auxotrophic Escherichia coli: antibodies for orotate decarboxylation.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Dissection of an antibody-catalyzed reaction.Proceedings of the National Academy of Sciences of the United States of America · 1994Review
- Secretion of a functional single-chain Fv protein in transgenic tobacco plants and cell suspension cultures.Plant molecular biology · 1993Article
- Binding of a high-energy substrate conformer in antibody catalysis.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- A new use for intracellular antibody expression: inactivation of human immunodeficiency virus type 1.Proceedings of the National Academy of Sciences of the United States of America · 1993Review
- A genetic approach to the generation of antibodies with enhanced catalytic activities.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Cellular and metabolic engineering. An overview.Applied biochemistry and biotechnologyReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have established a growth selection requirement for a catalytic antibody with modest chorismate mutase activity. Conversion of (-)-chorismate into prephenate is the key step in the biosynthesis of the aromatic amino acids tyrosine and phenylalanine. Strains of the yeast Saccharomyces cerevisiae containing an insertion mutation in the structural gene for the enzyme chorismate mutase (EC 5.4.99.5) require exogenous supplements of these two amino acids for efficient growth. Intracellular expression of the heterologous antibody catalyst in one such strain, identified by random mutagenesis and genetic selection, provides a substantial growth advantage under auxotrophic conditions; complementation was not observed with an unrelated esterolytic antibody. In addition to demonstrating that tailored immunoglobulin catalysts can carry out vital biochemical reactions in vivo, these experiments provide a powerful selection assay for identifying genetic changes within the antibody molecule itself that augment chemical efficiency.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.