ArticlePloS one2009
Sulfatide recognition by colonization factor antigen CS6 from enterotoxigenic Escherichia coli.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 54 citations in OpenAlex.
- Trial
- Emerging and Underdetected Colonization Factors in EnterotoxigenicMicroorganisms · 2026Review
- One Health Perspective on the EnterotoxigenicMicroorganisms · 2026Review
- An Asset for Food Safety: The Knowledge Behind the Physiological Alterations Induced by ETEC Enterotoxins.Foods (Basel, Switzerland) · 2025Review
- Beyond FimH: Diversity and Relevance of Carbohydrate-Binding Fimbrial Proteins in Escherichia coli.Chembiochem : a European journal of chemical biology · 2025Review
- Glycan-mediated adhesion mechanisms in antibiotic-resistant bacteria.BBA advances · 2025Article
- Colonizing and Virulence Factors in Enterotoxigenic Escherichia coli from Peru.The American journal of tropical medicine and hygiene · 2023Article
- The role of CFA/I in adherence and toxin delivery by ETEC expressing multiple colonization factors in the human enteroid model.PLoS neglected tropical diseases · 2022Article
- Sequence variations in the ETEC CS6 operon affect transcript and protein expression.Virulence · 2021Article
- Type II NKT Cell Agonist, Sulfatide, Is an Effective Adjuvant for Oral Heat-Killed Cholera Vaccines.Vaccines · 2021Article
- Colonization factor CS30 from enterotoxigenicVirulence · 2020Article
- Plasma lipid profiles discriminate bacterial from viral infection in febrile children.Scientific reports · 2019Article
- Biochemical and Immunological Evaluation of Recombinant CS6-Derived Subunit Enterotoxigenic Escherichia coli Vaccine Candidates.Infection and immunity · 2019Article
- Helicobacter pylori SabA binding gangliosides of human stomach.Virulence · 2018Article
- Interplay of a secreted protein with type IVb pilus for efficient enterotoxigenicProceedings of the National Academy of Sciences of the United States of America · 2018Article
- 3-Sulfogalactosyl-dependent adhesion ofThe Journal of biological chemistry · 2017Article
- Highly conserved type 1 pili promote enterotoxigenic E. coli pathogen-host interactions.PLoS neglected tropical diseases · 2017Article
- Article
- Polymorphonuclear leukocyte apoptosis is accelerated by sulfatides or sulfatides-treated Salmonella Typhimurium bacteria.BioMed research international · 2015Article
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The first step in the pathogenesis of enterotoxigenic Escherichia coli (ETEC) infections is adhesion of the bacterium to the small intestinal epithelium. Adhesion of ETEC is mediated by a number of antigenically distinct colonization factors, and among these, one of the most commonly detected is the non-fimbrial adhesin coli surface antigen 6 (CS6). The potential carbohydrate recognition by CS6 was investigated by binding of recombinant CS6-expressing E. coli and purified CS6 protein to a large number of variant glycosphingolipids separated on thin-layer chromatograms. Thereby, a highly specific binding of the CS6-expressing E. coli, and the purified CS6 protein, to sulfatide (SO(3)-3Galbeta1Cer) was obtained. The binding of the CS6 protein and CS6-expressing bacteria to sulfatide was inhibited by dextran sulfate, but not by dextran, heparin, galactose 4-sulfate or galactose 6-sulfate. When using recombinantly expressed and purified CssA and CssB subunits of the CS6 complex, sulfatide binding was obtained with the CssB subunit, demonstrating that the glycosphingolipid binding capacity of CS6 resides within this subunit. CS6-binding sulfatide was present in the small intestine of species susceptible to CS6-mediated infection, e.g. humans and rabbits, but lacking in species not affected by CS6 ETEC, e.g. mice. The ability of CS6-expressing ETEC to adhere to sulfatide in target small intestinal epithelium may thus contribute to virulence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.