ArticlePlant cell reports2009
Expression of a transgene exchanged by the recombinase-mediated cassette exchange (RMCE) method in plants.
Article in Plant cell reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed.
- Sequence modification on demand: search and replace tools for precise gene editing in plants.Transgenic research · 2021Review
- Recombinase-mediated integration of a multigene cassette in rice leads to stable expression and inheritance of the stacked locus.Plant direct · 2020Article
- Complex Trait Loci in Maize Enabled by CRISPR-Cas9 Mediated Gene Insertion.Frontiers in plant science · 2020Article
- High efficiency Agrobacterium-mediated site-specific gene integration in maize utilizing the FLP-FRT recombination system.Plant biotechnology journal · 2019Article
- Small serine recombination systems ParA-MRS and CinH-RS2 perform precise excision of plastid DNA.Plant biotechnology journal · 2017Article
- Progress of targeted genome modification approaches in higher plants.Plant cell reports · 2016Review
- Gene stacking by recombinases.Plant biotechnology journal · 2016Review
- Gene stacking in plant cell using recombinases for gene integration and nucleases for marker gene deletion.BMC biotechnology · 2015Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- Enrichments of gene replacement events byMolecular breeding : new strategies in plant improvementArticle
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a site-directed integration (SDI) system for Agrobacterium-mediated transformation to precisely integrate a single copy of a desired gene into a predefined target locus by recombinase-mediated cassette exchange (RMCE). We produced site-specific transgenic tobacco plants from four target lines and examined expression of the transgene in T1 site-specific transgenic tobacco plants, which were obtained by backcrossing. We found that site-specific transgenic plants from the same target lines showed approximately the same level of expression of the transgene. Moreover, we demonstrated that site-specific transgenic plants showed much less variability of transgene expression than random-integration transgenic plants. Interestingly, transgenes in the same direction at the same target locus showed the same level of activity, but transgenes in different directions showed different levels of activity. The expression levels of transgene did not correlate with those of the target gene. Our results showed that the SDI system could benefit the precise comparisons between different gene constructs, the characterization of different chromosomal regions and the cost-effective screening of reliable transgenic plants.
Indexed as
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.