ReviewMethods in molecular biology (Clifton, N.J.)2009
Use of stable isotope labeling by amino acids in cell culture (SILAC) for phosphotyrosine protein identification and quantitation.
Review in Methods in molecular biology (Clifton, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Mass Spectrometry for Proteomics-Based Investigation.Advances in experimental medicine and biology · 2026Review
- Highly Sensitive Detection of Tyrosine and Neurotransmitters by Stereoselective Biosynthesis and Photochemically Induced Dynamic Nuclear Polarization.Journal of the American Chemical Society · 2025Article
- Comprehensive stable-isotope tracing of glucose and amino acids identifies metabolic by-products and their sources in CHO cell culture.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A Tutorial Review of Labeling Methods in Mass Spectrometry-Based Quantitative Proteomics.ACS measurement science au · 2024Review
- Recent Contributions of Proteomics to Our Understanding of Reversible NJournal of proteome research · 2024Review
- Measuring Interactions Between Proteins and Small Molecules or Nucleic Acids.Current protocols · 2024Review
- Proteomics of the heart.Physiological reviews · 2024Review
- Proteomics insights into fragile X syndrome: Unraveling molecular mechanisms and therapeutic avenues.Neurobiology of disease · 2024Review
- Phosphoproteomics: a valuable tool for uncovering molecular signaling in cancer cells.Expert review of proteomics · 2021Review
- TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis.Journal of visualized experiments : JoVE · 2020Article
- Quantitative Comparison of Proteomes Using SILAC.Current protocols in protein science · 2019Review
- Bacterial production of site specificJournal of biomolecular NMR · 2017Article
- Physicochemical properties that control protein aggregation also determine whether a protein is retained or released from necrotic cells.Open biology · 2016Article
- Rapid enzyme regeneration results in the striking catalytic longevity of an engineered, single species, biocatalytic biofilm.Microbial cell factories · 2016Article
- Quantitative phosphotyrosine profiling of patient-derived xenografts identifies therapeutic targets in pediatric leukemia.Cancer research · 2016Article
- Systematic parameter estimation in data-rich environments for cell signalling dynamics.Bioinformatics (Oxford, England) · 2013Article
- Mass Spectrometry-based Proteomics and Peptidomics for Systems Biology and Biomarker Discovery.Frontiers in biology · 2012Article
- Chemical and biochemical approaches in the study of histone methylation and demethylation.Medicinal research reviews · 2012Review
- Phosphoproteomics in cancer.Molecular oncology · 2010Review
- Quantitative phosphoproteomic analysis of prion-infected neuronal cells.Cell communication and signaling : CCS · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In recent years, stable isotope labeling by amino acids in cell culture (SILAC) has become increasingly popular as a quantitative proteomic method. In SILAC experiments, proteins are metabolically labeled by culturing cells in media containing normal and heavy isotope amino acids. This makes proteins from the light and heavy cells distinguishable by mass spectrometry (MS) after the cell lysates are mixed and the proteins separated and/or enriched. SILAC is a powerful tool for the study of intracellular signal transduction. In particular, it has been very popular and successful in quantitative analysis of phosphotyrosine (pTyr) proteomes to characterize pTyr-dependent signaling pathways. In this chapter, we describe the SILAC procedure and use EphB signaling pathway as an example to illustrate the use of SILAC to investigate such pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.