ArticleJournal of cell science2009
Identification of a palmitoyl acyltransferase required for protein sorting to the flagellar membrane.
Article in Journal of cell science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 70 citations in OpenAlex.
- Importin α regulates ciliogenesis and cilia length with implications for Xenopus nephrogenesis.Journal of cell science · 2025Article
- Protein subcellular relocalization and function of duplicated flagellar calcium binding protein genes in honey bee trypanosomatid parasite.PLoS genetics · 2024Article
- Neuronal primary cilia integrate peripheral signals with metabolic drives.Frontiers in physiology · 2023Review
- PCARE requires coiled coil, RP62 kinase-binding and EVH1 domain-binding motifs for ciliary expansion.Human molecular genetics · 2022Article
- Touching the Surface: Diverse Roles for the Flagellar Membrane in Kinetoplastid Parasites.Microbiology and molecular biology reviews : MMBR · 2020Review
- Establishing and regulating the composition of cilia for signal transduction.Nature reviews. Molecular cell biology · 2019Review
- Lipid Modifications in Cilia Biology.Journal of clinical medicine · 2019Review
- Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes.Memorias do Instituto Oswaldo Cruz · 2018Article
- Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation.The Journal of biological chemistry · 2017Article
- The Lipid Raft Proteome of African Trypanosomes Contains Many Flagellar Proteins.Pathogens (Basel, Switzerland) · 2017Article
- Sterol targeting drugs reveal life cycle stage-specific differences in trypanosome lipid rafts.Scientific reports · 2017Article
- Dynamic protein S-palmitoylation mediates parasite life cycle progression and diverse mechanisms of virulence.Critical reviews in biochemistry and molecular biology · 2017Review
- Open Sesame: How Transition Fibers and the Transition Zone Control Ciliary Composition.Cold Spring Harbor perspectives in biology · 2017Review
- Global Profiling and Inhibition of Protein Lipidation in Vector and Host Stages of the Sleeping Sickness ParasiteACS infectious diseases · 2016Article
- Validation of N-myristoyltransferase as Potential Chemotherapeutic Target in Mammal-Dwelling Stages of Trypanosoma cruzi.PLoS neglected tropical diseases · 2016Article
- Quantitative Proteomics Uncovers Novel Factors Involved in Developmental Differentiation of Trypanosoma brucei.PLoS pathogens · 2016Article
- Flagellar membrane fusion and protein exchange in trypanosomes; a new form of cell-cell communication?F1000Research · 2016Article
- Architecture of a Host-Parasite Interface: Complex Targeting Mechanisms Revealed Through Proteomics.Molecular & cellular proteomics : MCP · 2015Article
- Ca2+ Regulation of Trypanosoma brucei Phosphoinositide Phospholipase C.Eukaryotic cell · 2015Article
- The Flagellar Arginine Kinase in Trypanosoma brucei Is Important for Infection in Tsetse Flies.PloS one · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Protein palmitoylation has diverse effects in regulating protein membrane affinity, localization, binding partner interactions, turnover and function. Here, we show that palmitoylation also contributes to the sorting of proteins to the eukaryotic flagellum. African trypanosomes are protozoan pathogens that express a family of unique Ca(2+)-binding proteins, the calflagins, which undergo N-terminal myristoylation and palmitoylation. The localization of calflagins depends on their acylation status. Myristoylation alone is sufficient for membrane association, but, in the absence of palmitoylation, the calflagins localize to the pellicular (cell body) membrane. Palmitoylation, which is mediated by a specific palmitoyl acyltransferase, is then required for subsequent trafficking of calflagin to the flagellar membrane. Coincident with the redistribution of calflagin from the pellicular to the flagellar membrane is their association with lipid rafts, which are highly enriched in the flagellar membrane. Screening of candidate palmitoyl acyltranferases identified a single enzyme, TbPAT7, that is necessary for calflagin palmitoylation and flagellar membrane targeting. Our results implicate protein palmitoylation in flagellar trafficking, and demonstrate the conservation and specificity of palmitoyl acyltransferase activity by DHHC-CRD proteins across kingdoms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.