ArticleThe Journal of biological chemistry2009
Calpain hydrolysis of alpha- and beta2-adaptins decreases clathrin-dependent endocytosis and may promote neurodegeneration.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Calpain cleaves the distal carboxyl terminus of TRPV1 and modulates its tachyphylaxis.Cell & bioscience · 2026Article
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- Real time imaging of intra-axonal calcium flux in an explant mouse model of axonal Guillain-Barré syndrome.Experimental neurology · 2022Article
- Alpha adaptins show isoform-specific association with neurofibrillary tangles in Alzheimer's disease.Neuropathology and applied neurobiology · 2022Article
- Synaptic tau: A pathological or physiological phenomenon?Acta neuropathologica communications · 2021Review
- Excitotoxicity-induced endocytosis as a potential target for stroke neuroprotection.Neural regeneration research · 2021Article
- Cerebral blood flow decrease as an early pathological mechanism in Alzheimer's disease.Acta neuropathologica · 2020Review
- Inhibiting calpain 1 and 2 in cyclin G associated kinase-knockout mice mitigates podocyte injury.JCI insight · 2020Article
- Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of nimesulide.Scientific reports · 2020Article
- The lipid phosphatase Synaptojanin 1 undergoes a significant alteration in expression and solubility and is associated with brain lesions in Alzheimer's disease.Acta neuropathologica communications · 2020Article
- Neuroprotective effect of grape seed extract on brain ischemia: a proteomic approach.Metabolic brain disease · 2019Article
- Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs.Proteome science · 2019Article
- Profiling of Signaling Proteins in Penumbra After Focal Photothrombotic Infarct in the Rat Brain Cortex.Molecular neurobiology · 2017Article
- Putting a brake on synaptic vesicle endocytosis.Cellular and molecular life sciences : CMLS · 2017Review
- Thrombin-Induced Calpain Activation Promotes Protease-Activated Receptor 1 Internalization.International journal of cell biology · 2017Article
- The Autophagy Machinery: A New Player in Chemotactic Cell Migration.Frontiers in neuroscience · 2017Review
- Chemotactic G protein-coupled receptors control cell migration by repressing autophagosome biogenesis.Autophagy · 2016Article
- The Role of PICALM in Alzheimer's Disease.Molecular neurobiology · 2015Review
- Global analysis of S-nitrosylation sites in the wild type (APP) transgenic mouse brain-clues for synaptic pathology.Molecular & cellular proteomics : MCP · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clathrin-dependent endocytosis is mediated by a tightly regulated network of molecular interactions that provides essential protein-protein and protein-lipid binding activities. Here we report the hydrolysis of the alpha- and beta2-subunits of the tetrameric adaptor protein complex 2 by calpain. Calcium-dependent alpha- and beta2-adaptin hydrolysis was observed in several rat tissues, including brain and primary neuronal cultures. Neuronal alpha- and beta2-adaptin cleavage was inducible by glutamate stimulation and was accompanied by the decreased endocytosis of transferrin. Heterologous expression of truncated forms of the beta2-adaptin subunit significantly decreased the membrane recruitment of clathrin and inhibited clathrin-mediated receptor endocytosis. Moreover, the presence of truncated beta2-adaptin sensitized neurons to glutamate receptor-mediated excitotoxicity. Proteolysis of alpha- and beta2-adaptins, as well as the accessory clathrin adaptors epsin 1, adaptor protein 180, and the clathrin assembly lymphoid myeloid leukemia protein, was detected in brain tissues after experimentally induced ischemia and in cases of human Alzheimer disease. The present study further clarifies the central role of calpain in regulating clathrin-dependent endocytosis and provides evidence for a novel mechanism through which calpain activation may promote neurodegeneration: the sensitization of cells to glutamate-mediated excitotoxicity via the decreased internalization of surface receptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.