ArticleThe Journal of biological chemistry2009
The sensor kinase TodS operates by a multiple step phosphorelay mechanism involving two autokinase domains.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Engineering NIR-sighted bacteria.eLife · 2025Article
- Genetic Analysis Reveals a Requirement for the Hybrid Sensor Kinase RscS inJournal of bacteriology · 2023Article
- Fluoro-recognition: New in vivo fluorescent assay for toluene dioxygenase probing induction by and metabolism of polyfluorinated compounds.Environmental microbiology · 2022Article
- Review
- Transcriptional Regulation of the Peripheral Pathway for the Anaerobic Catabolism of Toluene andFrontiers in microbiology · 2018Article
- New family of biosensors for monitoring BTX in aquatic and edaphic environments.Microbial biotechnology · 2016Article
- Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System.The Journal of biological chemistry · 2016Article
- Multiple signals modulate the activity of the complex sensor kinase TodS.Microbial biotechnology · 2015Article
- A tale of two machines: a review of the BLAST meeting, Tucson, AZ, 20-24 January 2013.Molecular microbiology · 2014Review
- Construction and application of an Escherichia coli bioreporter for aniline and chloroaniline detection.Journal of industrial microbiology & biotechnology · 2012Article
- Study of the TmoS/TmoT two-component system: towards the functional characterization of the family of TodS/TodT like systems.Microbial biotechnology · 2012Article
- The genetic organisation of prokaryotic two-component system signalling pathways.BMC genomics · 2010Article
- Catabolite repression of the TodS/TodT two-component system and effector-dependent transphosphorylation of TodT as the basis for toluene dioxygenase catabolic pathway control.Journal of bacteriology · 2010Article
- Article
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5 authors.
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Abstract
Expression of the Pseudomonas putida tod operon, which encodes enzymes for toluene metabolism, takes place from the P(todX) promoter and is mediated by the TodS/TodT two component system. The sensor kinase TodS has a complex domain arrangement containing two functional modules, each harboring a sensor- and an autokinase domain and separated by a receiver domain. Based on site-directed mutagenesis of phosphoaccepting His-190, Asp-500, and His-760 and in vitro transphosphorylation experiments with recombinant TodS fragments, we show that TodS uses a multiple step phosphorelay mechanism to activate TodT. Toluene binding stimulates exclusively phosphorylation of His-190, which is followed by phosphotransfer to Asp-500 and subsequently to His-760 prior to phosphorylation of TodT Asp-57. Mutation of His-190, Asp-500, and H760A prevented up-regulation of toluene-mediated stimulation of TodT transphosphorylation in vitro and reduced in vivo expression of P(todX) to the basal level. Calorimetric studies support that TodT binds to the C-terminal kinase module with a K(D) of approximately 200 nm and 1:1 stoichiometry. This is the first report of a multiple step phosphorelay mechanism of a sensor kinase that involves two autokinase domains.
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