ArticleThe Journal of biological chemistry2009
An alternatively spliced isoform of non-muscle myosin II-C is not regulated by myosin light chain phosphorylation.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
- A song of heads and tails: myosin II conformational regulation and filament dynamics shape force generation in non-muscle cells.Biophysical reviews · 2026Review
- Non-muscle myosin IIC predominantly expressed in the slow-twitch skeletal muscles impedes age-related muscle weakness.PloS one · 2025Article
- Structure, regulation, and mechanisms of nonmuscle myosin-2.Cellular and molecular life sciences : CMLS · 2024Review
- Nonmuscle myosin-2 contractility-dependent actin turnover limits the length of epithelial microvilli.Molecular biology of the cell · 2020Article
- The role of nonmuscle myosin 2A and 2B in the regulation of mesenchymal cell contact guidance.Molecular biology of the cell · 2019Article
- Replacing nonmuscle myosin 2A with myosin 2C1 permits gastrulation but not placenta vascular development in mice.Molecular biology of the cell · 2018Article
- Article
- A Perspective on the Role of Myosins as Mechanosensors.Biophysical journal · 2016Article
- Notochord morphogenesis in mice: Current understanding & open questions.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Review
- Mechanisms of Vascular Smooth Muscle Contraction and the Basis for Pharmacologic Treatment of Smooth Muscle Disorders.Pharmacological reviews · 2016Review
- Myosin 18A coassembles with nonmuscle myosin 2 to form mixed bipolar filaments.Current biology : CB · 2015Article
- The role of vertebrate nonmuscle Myosin II in development and human disease.Bioarchitecture · 2014Review
- Characterization of three full-length human nonmuscle myosin II paralogs.The Journal of biological chemistry · 2013Article
- Kinetic characterization of the ATPase and actin-activated ATPase activities of Acanthamoeba castellanii myosin-2.The Journal of biological chemistry · 2013Article
- Loss of actomyosin regulation in distal arthrogryposis myopathy due to mutant myosin binding protein-C slow.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Myosin motors at neuronal synapses: drivers of membrane transport and actin dynamics.Nature reviews. Neuroscience · 2013Review
- The effect of including the C2 insert of nonmuscle myosin II-C on neuritogenesis.The Journal of biological chemistry · 2013Article
- Nonmuscle myosin-2: mix and match.Cellular and molecular life sciences : CMLS · 2013Review
- In vivo studies on nonmuscle myosin II expression and function in heart development.Frontiers in bioscience (Landmark edition) · 2012Review
- Comparative kinetic and functional characterization of the motor domains of human nonmuscle myosin-2C isoforms.The Journal of biological chemistry · 2011Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
We report a novel isoform of non-muscle myosin II-C (NM II-C), NM II-C2, that is generated by alternative splicing of an exon, C2, encoding 41 amino acids in mice (33 in humans). The 41 amino acids are inserted into loop 2 of the NM II-C heavy chain within the actin binding region. Unlike most vertebrate non-muscle and smooth muscle myosin IIs, baculovirus-expressed mouse heavy meromyosin (HMM) II-C2 demonstrates no requirement for regulatory myosin light chain (MLC(20)) phosphorylation for maximum actin-activated MgATPase activity or maximum in vitro motility as measured by the sliding actin filament assay. In contrast, noninserted HMM II-C0 and another alternatively spliced isoform HMM II-C1, which contains 8 amino acids inserted into loop 1, are dependent on MLC(20) phosphorylation for both actin-activated MgATPase activity and in vitro motility ( Kim, K. Y., Kovacs, M., Kawamoto, S., Sellers, J. R., and Adelstein, R. S. (2005) J. Biol. Chem. 280, 22769-22775 ). HMM II-C1C2, which contains both the C1 and C2 inserts, does not require MLC(20) phosphorylation for full activity similar to HMM II-C2. These constitutively active C2-inserted isoforms of NM II-C are expressed only in neuronal tissue. This is in contrast to NM II-C1 and NM II-C0, both of which are ubiquitously expressed. Full-length NM II-C2-GFP expressed in COS-7 cells localizes to filaments in interphase cells and to the cytokinetic ring in dividing cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.