ArticleBMC molecular biology2009
Binding of PFOS to serum albumin and DNA: insight into the molecular toxicity of perfluorochemicals.
Article in BMC molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 115 citations in OpenAlex.
- Associations of Perfluoroalkyl substances with blood lipids and Apolipoproteins in lipoprotein subspecies: the POUNDS-lost study.Environmental health : a global access science source · 2020Trial
- Review
- Toward Comprehensive In Vitro Evaluation of Serum Albumin Binding of Per- and Polyfluoroalkyl Substances.Journal of xenobiotics · 2026Review
- The Impact of Forever Chemicals on Protein Structure and Function.International journal of molecular sciences · 2026Review
- Review
- Bovine Serum Albumin-Based Sponges as Biocompatible Adsorbents: Development, Characterization, and Perfluorooctane Sulfonate Removal Efficiency.Small science · 2025Article
- Impact of PFOS Exposure on Murine Fetal Hematopoietic Stem Cells, Associated with Intrauterine Metabolic Perturbation.Environmental science & technology · 2025Article
- Quantitative cross-species comparison of serum albumin binding of per- and polyfluoroalkyl substances from five structural classes.Toxicological sciences : an official journal of the Society of Toxicology · 2024Article
- Screening Peptide-Binding Partners for GenX via Phage Display.International journal of molecular sciences · 2024Article
- Article
- Insights into the enzymatic degradation of DNA expedited by typical perfluoroalkyl acids.Eco-Environment & Health · 2023Article
- Probing the Interactions of Perfluorocarboxylic Acids of Various Chain Lengths with Human Serum Albumin: Calorimetric and Spectroscopic Investigations.Chemical research in toxicology · 2023Article
- Computational Analysis of the Binding Mechanism of GenX and HSA.ACS omega · 2021Article
- Potency Ranking of Per- and Polyfluoroalkyl Substances Using High-Throughput Transcriptomic Analysis of Human Liver Spheroids.Toxicological sciences : an official journal of the Society of Toxicology · 2021Article
- Sensors for detecting per- and polyfluoroalkyl substances (PFAS): A critical review of development challenges, current sensors, and commercialization obstacles.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2021Article
- Binding of Per- and Polyfluoro-alkyl Substances to Peroxisome Proliferator-Activated Receptor Gamma.ACS omega · 2021Article
- Unveiling the binding mode of perfluorooctanoic acid to human serum albumin.Protein science : a publication of the Protein Society · 2021Article
- Article
- Dominant entropic binding of perfluoroalkyl substances (PFASs) to albumin protein revealed byChemosphere · 2021Article
- Assessment of the Emerging Threat Posed by Perfluoroalkyl and Polyfluoroalkyl Substances to Male Reproduction in Humans.Frontiers in endocrinology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHealth risk from exposure of perfluorochemicals (PFCs) to wildlife and human has been a subject of great interest for understanding their molecular mechanism of toxicity. Although much work has been done, the toxigenicity of PFCs remains largely unknown. In this work, the non-covalent interactions between perfluorooctane sulfonate (PFOS) and serum albumin (SA) and DNA were investigated under normal physiological conditions, aiming to elucidate the toxigenicity of PFCs.
resultsIn equilibrium dialysis assay, the bindings of PFOS to SA correspond to the Langmuir isothermal model with two-step sequence model. The saturation binding number of PFOS was 45 per molecule of SA and 1 per three base-pairs of DNA, respectively. ITC results showed that all the interactions were spontaneous driven by entropy change. Static quenching of the fluorescence of SA was observed when interacting with PFOS, indicating PFOS bound Trp residue of SA. CD spectra of SA and DNA changed obviously in the presence of PFOS. At normal physiological conditions, 1.2 mmol/l PFOS reduces the binding ratio of Vitamin B2 to SA by more than 30%.
conclusionThe ion bond, van der Waals force and hydrophobic interaction contributed to PFOS binding to peptide chain of SA and to the groove bases of DNA duplex. The non-covalent interactions of PFOS with SA and DNA alter their secondary conformations, with the physiological function of SA to transport Vitamin B2 being inhibited consequently. This work provides a useful experimental method for further studying the toxigenicity of PFCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.