ArticleBiomacromolecules2009
Cytosolic delivery mediated via electrostatic surface binding of protein, virus, or siRNA cargos to pH-responsive core-shell gel particles.
Article in Biomacromolecules, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 73 citations in OpenAlex.
- Protein charge parameters that influence stability and cellular internalization of polyelectrolyte complex micelles.Chemical science · 2022Article
- A Combination Therapy Using Electrical Stimulation and Adaptive, Conductive Hydrogels Loaded with Self-Assembled Nanogels Incorporating Short Interfering RNA Promotes the Repair of Diabetic Chronic Wounds.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Smart pH-responsive nanomedicines for disease therapy.Journal of pharmaceutical investigation · 2022Review
- Metal Sulfide Semiconductor Nanomaterials and Polymer Microgels for Biomedical Applications.International journal of molecular sciences · 2021Review
- Targeting the Inside of Cells with Biologicals: Chemicals as a Delivery Strategy.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
- Lipopolysaccharide-affinity copolymer senses the rapid motility of swarmer bacteria to trigger antimicrobial drug release.Nature communications · 2018Article
- Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment.Chemical reviews · 2017Review
- One-pot synthesis of pH-responsive hybrid nanogel particles for the intracellular delivery of small interfering RNA.Biomaterials · 2016Article
- Synthetic Nanoparticles for Vaccines and Immunotherapy.Chemical reviews · 2015Review
- Stimulus-responsive hydrogels: Theory, modern advances, and applications.Materials science & engineering. R, Reports : a review journal · 2015Article
- Stimuli-responsive nanomaterials for therapeutic protein delivery.Journal of controlled release : official journal of the Controlled Release Society · 2014Review
- Materials based tumor immunotherapy vaccines.Current opinion in immunology · 2013Review
- Applications of polymeric adjuvants in studying autoimmune responses and vaccination against infectious diseases.Journal of the Royal Society, Interface · 2013Review
- Biomaterials-based modulation of the immune system.BioMed research international · 2013Review
- Nonendocytic delivery of functional engineered nanoparticles into the cytoplasm of live cells using a novel, high-throughput microfluidic device.Nano letters · 2012Article
- Engineering nano- and microparticles to tune immunity.Advanced materials (Deerfield Beach, Fla.) · 2012Review
- Strategies for ocular siRNA delivery: Potential and limitations of non-viral nanocarriers.Journal of biological engineering · 2012Article
- Reductively responsive siRNA-conjugated hydrogel nanoparticles for gene silencing.Journal of the American Chemical Society · 2012Article
- Multifunctional core-shell nanoparticles: discovery of previously invisible biomarkers.Journal of the American Chemical Society · 2011Article
- Controlled protein delivery based on enzyme-responsive nanocapsules.Advanced materials (Deerfield Beach, Fla.) · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
We recently described a strategy for intracellular delivery of macromolecules, utilizing pH-responsive "core-shell" structured gel particles. These cross-linked hydrogel particles disrupt endosomes with low toxicity by virtue of physical sequestration of an endosome-disrupting "proton sponge" core inside a nontoxic hydrophilic shell. Here we tested the efficacy of this system for cytosolic delivery of a broad range of macromolecular cargos, and demonstrate the delivery of proteins, whole viral particles, or siRNA oligonucleotides into the cytosol of dendritic cells and epithelial cells via core-shell particles. We assessed the functional impact of particle delivery for vaccine applications and found that cytosolic delivery of protein antigens in dendritic cells via the core-shell particles promotes priming of CD8(+) T-cells at 100-fold lower doses than soluble protein. Functional gene knockdown following delivery of siRNA using the particles was demonstrated in epithelial cells. Based on these findings, these materials may be of interest for a broad range of biomedical applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.