ArticlePlant molecular biology2009
Plant-specific SR-related protein atSR45a interacts with spliceosomal proteins in plant nucleus.
Article in Plant molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Splicing regulation by RS2Z36 controls ovary patterning and fruit growth in tomato.Plant molecular biology · 2026Article
- The Regulatory Roles of RNA-Binding Proteins in Plant Salt Stress Response.Plants (Basel, Switzerland) · 2025Review
- Article
- Concordant Gene Expression and Alternative Splicing Regulation under Abiotic Stresses in Arabidopsis.Genes · 2024Article
- The Splicing Factor SR45 Negatively Regulates Anthocyanin Accumulation under High-Light Stress inLife (Basel, Switzerland) · 2023Article
- ABA signaling prevents phosphodegradation of the SR45 splicing factor to alleviate inhibition of early seedling development in Arabidopsis.Plant communications · 2023Article
- Alternative splicing in ABA signaling during seed germination.Frontiers in plant science · 2023Review
- Regulatory Network of Serine/Arginine-Rich (SR) Proteins: The Molecular Mechanism and Physiological Function in Plants.International journal of molecular sciences · 2022Review
- Relevance and Regulation of Alternative Splicing in Plant Heat Stress Response: Current Understanding and Future Directions.Frontiers in plant science · 2022Review
- Transcriptomic and splicing changes underlying tomato responses to combined water and nutrient stress.Frontiers in plant science · 2022Article
- SUMO Modification of OsFKBP20-1b Is Integral to Proper Pre-mRNA Splicing upon Heat Stress in Rice.International journal of molecular sciences · 2021Article
- Daily temperature cycles promote alternative splicing of RNAs encoding SR45a, a splicing regulator in maize.Plant physiology · 2021Article
- Plant responses to high temperature: a view from pre-mRNA alternative splicing.Plant molecular biology · 2021Review
- Identification and Regulation of Tomato Serine/Arginine-Rich Proteins Under High Temperatures.Frontiers in plant science · 2021Article
- The Arabidopsis lncRNA ASCO modulates the transcriptome through interaction with splicing factors.EMBO reports · 2020Article
- Role of MPK4 in pathogen-associated molecular pattern-triggered alternative splicing in Arabidopsis.PLoS pathogens · 2020Article
- Light Regulation of Alternative Pre-mRNA Splicing in Plants.Photochemistry and photobiology · 2017Review
- Pre-mRNA Splicing in Plants: In Vivo Functions of RNA-Binding Proteins Implicated in the Splicing Process.Biomolecules · 2015Review
- The PRP6-like splicing factor STA1 is involved in RNA-directed DNA methylation by facilitating the production of Pol V-dependent scaffold RNAs.Nucleic acids research · 2013Article
- Unraveling the circadian clock in Arabidopsis.Plant signaling & behavior · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serine/arginine-rich (SR) protein and its homologues (SR-related proteins) are important regulators of constitutive and/or alternative splicing and other aspects of mRNA metabolism. To clarify the contribution of a plant-specific and stress-responsive SR-related protein, atSR45a, to splicing events, here we analyzed the interaction of atSR45a with the other splicing factors by conducting a yeast two-hybrid assay and a bimolecular fluorescence complementation analysis. The atSR45a-1a and -2 proteins, the presumed mature forms produced by alternative splicing of atSR45a, interacted with U1-70K and U2AF(35)b, splicing factors for the initial definition of 5' and 3' splice sites, respectively, in the early stage of spliceosome assembly. Both proteins also interacted with themselves, other SR proteins (atSR45 and atSCL28), and PRP38-like protein, a homologue of the splicing factor essential for cleavage of the 5' splice site. The mapping of deletion mutants of atSR45a proteins revealed that the C-terminal arginine/serine-rich (RS) domain of atSR45a proteins are required for the interaction with U1-70K, U2AF(35)b, atSR45, atSCL28, PRP38-like protein, and themselves, and the N-terminal RS domain enhances the interaction efficiency. Interestingly, the distinctive N-terminal extension in atSR45a-1a protein, but not atSR45a-2 protein, inhibited the interaction with these splicing factors. These findings suggest that the atSR45a proteins help to form the bridge between 5' and 3' splice sites in the spliceosome assembly and the efficiency of spliceosome formation is affected by the expression ratio of atSR45a-1a and atSR45a-2.
Indexed as
Identifiers
19238562What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.