ArticleCurrent genetics2009
The BEM46-like protein appears to be essential for hyphal development upon ascospore germination in Neurospora crassa and is targeted to the endoplasmic reticulum.
Article in Current genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- A user-friendly CRISPR/Cas9 system for mutagenesis of Neurospora crassa.Scientific reports · 2024Article
- Complementation of an Eisosomal YeastJournal of fungi (Basel, Switzerland) · 2023Article
- Discovery of ER-localized sugar transporters for cellulase production with lac1 being essential.Biotechnology for biofuels and bioproducts · 2022Article
- Activity-based protein profiling of human andiScience · 2022Article
- The Antimalarial Natural Product Salinipostin A Identifies Essential α/β Serine Hydrolases Involved in Lipid Metabolism in P. falciparum Parasites.Cell chemical biology · 2020Article
- The Composition and the Structure of MCC/Eisosomes inFrontiers in microbiology · 2020Article
- BEM46 shows eisosomal localization and association with tryptophan-derived auxin pathway in Neurospora crassa.Eukaryotic cell · 2014Article
- Characterization of a serine hydrolase targeted by acyl-protein thioesterase inhibitors in Toxoplasma gondii.The Journal of biological chemistry · 2013Article
- From complete genome sequence to 'complete' understanding?Trends in biotechnology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bud emergence (BEM)46 proteins are evolutionarily conserved members of the alpha/beta-hydrolase super family, but their exact role remains unknown. To better understand the cellular role of BEM46 and its homologs, we used the model organism Neurospora crassa in conjunction with bem46 RNAi, over-expression vectors, and repeat induced point mutation analyzes. We clearly demonstrated that BEM46 is required for cell type-specific hyphal growth, which indicates a role for BEM46 in maintaining polarity. Vegetative hyphae, perithecia, and ascospores developed normally, but hyphae germinating from ascospores exhibited a loss-of-polarity phenotype. We also found that the BEM46 protein is targeted to the perinuclear endoplasmic reticulum (ER) and also localizes at or close to the plasma membrane. Our findings show that BEM46 can be used as a new ER marker for filamentous fungi, the first for N. crassa. Our data suggest that BEM46 plays a role in a signal transduction pathway involved in determining or maintaining cell type-specific polarity. This implies a higher degree of fungal hyphae differentiation than previously expected. This work also has implications for higher eukaryotic cells with polarized growth, such as pollen tubes or neuronal cells.
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