ArticlePloS one2009
A novel immunological assay for hepcidin quantification in human serum.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 85 citations in OpenAlex.
- Hepcidin as a Molecular Hub of Iron Homeostasis: From BMP-SMAD Signaling to Therapeutic Modulation.Biomolecules · 2026Review
- Measurement of serum hepcidin-25 by latex agglutination in healthy volunteers and patients with hematologic disorders.International journal of hematology · 2024Article
- Hepcidin as a Potential Biomarker for the Diagnosis of Anemia.Turkish journal of pharmaceutical sciences · 2022Article
- Serum Procalcitonin Levels in Newly Diagnosed Hodgkin Lymphoma: Correlation with Other Inflammatory Biomarkers.Medicina (Kaunas, Lithuania) · 2022Article
- Tuberculosis-Associated Anemia: A Narrative Review.Cureus · 2022Review
- Clinical interpretation of serum hepcidin-25 in inflammation and renal dysfunction.Journal of mass spectrometry and advances in the clinical lab · 2022Article
- Article
- The Hepatitis C virus NS5A and core proteins exert antagonistic effects on HAMP gene expression: the hidden interplay with the MTF-1/MRE pathway.FEBS open bio · 2021Article
- Green tea activity and iron overload induced molecular fibrogenesis of rat liver.Saudi journal of biological sciences · 2019Article
- Rapid elevation of transferrin saturation and serum hepcidin concentration in hemodialysis patients after intravenous iron infusion.Haematologica · 2015Article
- Article
- New insights on iron study in myelodysplasia.Turkish journal of haematology : official journal of Turkish Society of Haematology · 2014Article
- Nanopore film based enrichment and quantification of low abundance hepcidin from human bodily fluids.Nanomedicine : nanotechnology, biology, and medicine · 2014Article
- Hepcidin production in response to iron is controlled by monocyte-derived humoral factors.International journal of hematology · 2014Article
- Iron regulation by hepcidin.The Journal of clinical investigation · 2013Article
- Inhibiting heme oxygenase-1 attenuates rat liver fibrosis by removing iron accumulation.World journal of gastroenterology · 2013Article
- Diagnosis of anaemia: old things rearranged.Wiener medizinische Wochenschrift (1946) · 2012Review
- Serum hepcidin as a diagnostic test of iron deficiency in premenopausal female blood donors.Haematologica · 2011Article
- A novel monoclonal antibody immunoassay for the detection of human serum hepcidin.Journal of gastroenterology · 2011Article
- Mammalian target of rapamycin (mTOR) inhibitors: potential uses and a review of haematological adverse effects.Drug safety · 2011Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHepcidin is a 25-aminoacid cysteine-rich iron regulating peptide. Increased hepcidin concentrations lead to iron sequestration in macrophages, contributing to the pathogenesis of anaemia of chronic disease whereas decreased hepcidin is observed in iron deficiency and primary iron overload diseases such as hereditary hemochromatosis. Hepcidin quantification in human blood or urine may provide further insights for the pathogenesis of disorders of iron homeostasis and might prove a valuable tool for clinicians for the differential diagnosis of anaemia. This study describes a specific and non-operator demanding immunoassay for hepcidin quantification in human sera. METHODS AND
findingsAn ELISA assay was developed for measuring hepcidin serum concentration using a recombinant hepcidin25-His peptide and a polyclonal antibody against this peptide, which was able to identify native hepcidin. The ELISA assay had a detection range of 10-1500 microg/L and a detection limit of 5.4 microg/L. The intra- and interassay coefficients of variance ranged from 8-15% and 5-16%, respectively. Mean linearity and recovery were 101% and 107%, respectively. Mean hepcidin levels were significantly lower in 7 patients with juvenile hemochromatosis (12.8 microg/L) and 10 patients with iron deficiency anemia (15.7 microg/L) and higher in 7 patients with Hodgkin lymphoma (116.7 microg/L) compared to 32 age-matched healthy controls (42.7 microg/L).
conclusionsWe describe a new simple ELISA assay for measuring hepcidin in human serum with sufficient accuracy and reproducibility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.