ArticleNucleic acids research1991
Coregulation of the Kluyveromyces lactis lactose permease and beta-galactosidase genes is achieved by interaction of multiple LAC9 binding sites in a 2.6 kbp divergent promoter.
Article in Nucleic acids research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- A unique metabolic gene cluster regulates lactose and galactose metabolism in the yeastApplied and environmental microbiology · 2024Article
- Repeated Cis-Regulatory Tuning of a Metabolic Bottleneck Gene during Evolution.Molecular biology and evolution · 2018Article
- Divergent Evolution of the Transcriptional Network Controlled by Snf1-Interacting Protein Sip4 in Budding Yeasts.PloS one · 2015Article
- Kinetics and regulation of lactose transport and metabolism in Kluyveromyces lactis JA6.World journal of microbiology & biotechnology · 2014Article
- Regulations of sugar transporters: insights from yeast.Current genetics · 2013Review
- A novel, lactase-based selection and strain improvement strategy for recombinant protein expression in Kluyveromyces lactis.Microbial cell factories · 2012Article
- Adaptive evolution of a lactose-consuming Saccharomyces cerevisiae recombinant.Applied and environmental microbiology · 2008Article
- Role of Snf1p in regulation of intracellular sorting of the lactose and galactose transporter Lac12p in Kluyveromyces lactis.Eukaryotic cell · 2005Article
- Feedback regulation of glucose transporter gene transcription in Kluyveromyces lactis by glucose uptake.Journal of bacteriology · 2001Article
- FacB, the Aspergillus nidulans activator of acetate utilization genes, binds dissimilar DNA sequences.The EMBO journal · 1998Article
- Gal80 proteins of Kluyveromyces lactis and Saccharomyces cerevisiae are highly conserved but contribute differently to glucose repression of the galactose regulon.Molecular and cellular biology · 1993Article
- Autoregulation of GAL4 transcription is essential for rapid growth of Kluyveromyces lactis on lactose and galactose.Nucleic acids research · 1993Article
- Expression of the transcriptional activator LAC9 (KlGAL4) in Kluyveromyces lactis is controlled by autoregulation.Molecular and cellular biology · 1993Article
- beta-Galactosidase of Kluyveromyces lactis (Lac4p) as reporter of gene expression in Candida albicans and C. tropicalis.Molecular & general genetics : MGG · 1992Article
- Review
- Identification of the lactose LAC gene superfamilies in Kluyveromyces yeast.Doklady. Biochemistry and biophysicsArticle
- The yeast Kluyveromyces lactis as an efficient host for heterologous gene expression.Antonie van LeeuwenhoekReview
- Domestication of dairy yeast Kluyveromyces lactis: transfer of the beta-galactosidase (LAC4) and lactose permease (LAC12) gene cluster?Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sectionsArticle
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4 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
The coregulated genes LAC4 and LAC12 encoding beta-galactosidase and lactose permease, respectively, are responsible for the ability of the milk yeast Kluyveromyces lactis to utilise lactose. They are divergently transcribed and separated by an unusually large intergenic region of 2.6 kbp. Mapping of the upstream border of the beta-galactosidase gene (LAC4) promoter by introduction of mutations at the chromosomal locus showed that LAC4 and LAC12 share the same upstream activation sites (UAS). The UASs represent binding sites for the trans-activator LAC9, a K. lactis homologue of GAL4, conforming to the consensus sequence 5'-CGG(N5)A/T(N5)CCG-3'. Two binding sites are located in front of each of the genes at almost symmetrical positions. beta-galactosidase activity measurements as well as quantitation of LAC4 and LAC12 mRNA levels demonstrated that all four sites are required for full induction. LAC4 proximal and a LAC12 proximal sites cooperate in activating transcription of both genes. These sites are more than 1.7 kbp apart and the distal site is located more than 2.3 kbp upstream of the respective start of transcription. Thus, the distance between interacting sites is larger than in any of the well characterised yeast promoters. The contribution to gene activation differs for individual binding sites and correlates with the relative affinity of LAC9 for these sites in vitro suggesting that LAC9 binding is a rate limiting step for LAC promoter function.
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