ArticleGenetics2009
Alternative induction of meiotic recombination from single-base lesions of DNA deaminases.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Light-controlled Spo11-less meiotic DNA breaks by MagTAQing lead to chromosomal aberrations.Nucleic acids research · 2025Article
- Chromosome-dependent aneuploid formation in Spo11-less meiosis.Genes to cells : devoted to molecular & cellular mechanisms · 2023Article
- Comparative Genomics Supports Sex and Meiosis in Diverse Amoebozoa.Genome biology and evolution · 2018Article
- Amoebozoans Are Secretly but Ancestrally Sexual: Evidence for Sex Genes and Potential Novel Crossover Pathways in Diverse Groups of Amoebae.Genome biology and evolution · 2017Article
- Recombination Is Responsible for the Increased Recovery of Drug-Resistant Mutants with Hypermutated Genomes in Resting Yeast Diploids Expressing APOBEC Deaminases.Frontiers in genetics · 2017Article
- Article
- Origins of eukaryotic sexual reproduction.Cold Spring Harbor perspectives in biology · 2014Review
- A mutation in the FHA domain of Coprinus cinereus Nbs1 Leads to Spo11-independent meiotic recombination and chromosome segregation.G3 (Bethesda, Md.) · 2013Article
- Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination.G3 (Bethesda, Md.) · 2013Article
- Meiotic recombination protein Rec12: functional conservation, crossover homeostasis and early crossover/non-crossover decision.Nucleic acids research · 2011Article
- Single-strand nicks induce homologous recombination with less toxicity than double-strand breaks using an AAV vector template.Nucleic acids research · 2011Article
- Non-homologous end joining plays a key role in transgene concatemer formation in transgenic zebrafish embryos.International journal of biological sciences · 2010Article
- Genomic signatures of germline gene expression.Genome research · 2010Article
- Uniting sex and eukaryote origins in an emerging oxygenic world.Biology direct · 2010Article
- Recruitment of MRE-11 to complex DNA damage is modulated by meiosis-specific chromosome organization.Mutation researchArticle
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Meiotic recombination enhances genetic diversity as well as ensures proper segregation of homologous chromosomes, requiring Spo11-initiated double-strand breaks (DSBs). DNA deaminases act on regions of single-stranded DNA and deaminate cytosine to uracil (dU). In the immunoglobulin locus, this lesion will initiate point mutations, gene conversion, and DNA recombination. To begin to delineate the effect of induced base lesions on meiosis, we analyzed the effect of expressing DNA deaminases (activation-induced deaminase, AID, and APOBEC3C) in germ cells. We show that meiotic dU:dG lesions can partially rescue a spo11Delta phenotype in yeast and worm. In rec12 Schizosaccharomyces pombe, AID expression increased proper chromosome segregation, thereby enhancing spore viability, and induced low-frequency meiotic crossovers. Expression of AID in the germ cells of Caenorhabditis elegans spo-11 induced meiotic RAD-51 foci formation and chromosomal bivalency and segregation, as well as an increase in viability. RNAi experiments showed that this rescue was dependent on uracil DNA-glycosylase (Ung). Furthermore, unlike ionizing radiation-induced spo-11 rescue, AID expression did not induce large numbers of DSBs during the rescue. This suggests that the products of DNA deamination and base excision repair, such as uracil, an abasic site, or a single-stranded nick, are sufficient to initiate and alter meiotic recombination in uni- and multicellular organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.