ArticleGenome research2009
Genomic mapping of binding regions for the Ecdysone receptor protein complex.
Article in Genome research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 106 citations in OpenAlex.
- Duplicated paralog of insulin receptor functions distinctively in locust reproduction for high fecundity.Insect science · 2026Article
- Launching the molecular genetic investigation of the ecdysone signaling cascade: a tribute to David S. Hogness (1925-2019).Genetics · 2025Article
- Remote control of AMPK via extracellular adenosine controls tissue growth.Nature cell biology · 2025Article
- Azadirachtin disrupts ecdysone signaling and alters sand fly immunity.Parasites & vectors · 2024Article
- Genetic characterization of candidate ecdysteroid kinases in Drosophila melanogaster.G3 (Bethesda, Md.) · 2024Article
- Inter-organ steroid hormone signaling promotes myoblast fusion via direct transcriptional regulation of a single key effector gene.Current biology : CB · 2024Article
- Phylogenomics of the Ecdysteroid Kinase-like (EcKL) Gene Family in Insects Highlights Roles in Both Steroid Hormone Metabolism and Detoxification.Genome biology and evolution · 2024Article
- Long-range repression by ecdysone receptor on complex enhancers of the insulin receptor gene.Fly · 2023Article
- Steroid hormone regulation of innate immunity in Drosophila melanogaster.PLoS genetics · 2023Review
- Long-range repression by ecdysone receptor on complex enhancers of the insulin receptor gene.bioRxiv : the preprint server for biology · 2023Article
- Developmental regulation of epithelial cell cuboidal-to-squamous transition in Drosophila follicle cells.Developmental biology · 2022Article
- Genome-wide transcriptomic changes reveal the genetic pathways involved in insect migration.Molecular ecology · 2022Article
- TheProceedings. Biological sciences · 2022Article
- Direct and indirect gene repression by the ecdysone cascade during mosquito reproductive cycle.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Cyromazine affects the ovarian germ cells ofFrontiers in physiology · 2022Article
- Anatomy and evolution of a DNA replication origin.Chromosoma · 2021Article
- Article
- Review
- Nuclear receptors linking physiology and germline stem cells in Drosophila.Vitamins and hormones · 2021Review
- Pri smORF Peptides Are Wide Mediators of Ecdysone Signaling, Contributing to Shape Spatiotemporal Responses.Frontiers in genetics · 2021Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We determined the physical locations of the heterodimeric Ecdysone receptor/Ultraspiracle (ECR/USP) nuclear hormone receptor complex throughout the entire nonrepetitive genome of Drosophila melanogaster using a cell line (Kc167) that differentiates in response to 20-hydroxyecdysone (20-HE). 20-HE, the natural ligand of this complex, controls major aspects of insect development, including molting, metamorphosis, and reproduction. Direct gene targets of 20-HE signaling were identified by combining this physical binding-site profiling with gene expression profiling after treatment with 20-HE. We found 502 significant regions of ECR/USP binding throughout the genome. Only 42% of these regions are nearby genes that are 20-HE responsive in these cells. However, at least three quarters of the remaining ECR/USP regions are near 20-HE-regulated genes in other tissue and cell types during metamorphosis, suggesting that binding at many regulatory elements in the genome is largely noncell-type specific. The majority (21/26) of the early targets of 20-HE encode transcriptional regulatory factors. To determine whether any of these targets are required for the morphological differentiation of these cells, we used RNAi to reduce the expression of each of the 26 early genes. Accordingly, we found that three direct targets of ECR/USP--hairy, vrille, and Hr4--are required for cellular differentiation in response to the hormone. Initial mutational analysis of vrille in vivo reveals that it is required for metamorphosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.