ArticleNucleic acids research2009
Novel sequencing strategy for repetitive DNA in a Drosophila BAC clone reveals that the centromeric region of the Y chromosome evolved from a telomere.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Centromere structure and function: lessons from Drosophila.Genetics · 2023Article
- Article
- The structure of an endogenous Drosophila centromere reveals the prevalence of tandemly repeated sequences able to form i-motifs.Scientific reports · 2015Article
- Long-Read Single Molecule Sequencing to Resolve Tandem Gene Copies: The Mst77Y Region on the Drosophila melanogaster Y Chromosome.G3 (Bethesda, Md.) · 2015Article
- The Release 6 reference sequence of the Drosophila melanogaster genome.Genome research · 2015Article
- Telomeres and centromeres have interchangeable roles in promoting meiotic spindle formation.The Journal of cell biology · 2015Article
- Interstitial telomeric repeats are enriched in the centromeres of chromosomes in Solanum species.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Article
- On the origin of the eukaryotic chromosome: the role of noncanonical DNA structures in telomere evolution.Genome biology and evolution · 2013Article
- The Subtelomeric khipu Satellite Repeat from Phaseolus vulgaris: Lessons Learned from the Genome Analysis of the Andean Genotype G19833.Frontiers in plant science · 2013Article
- Retrotransposons that maintain chromosome ends.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Transcriptional analysis of the HeT-A retrotransposon in mutant and wild type stocks reveals high sequence variability at Drosophila telomeres and other unusual features.BMC genomics · 2011Article
- Molecular and cytological characterization of repetitive DNA sequences from the centromeric heterochromatin of Sciara coprophila.Chromosoma · 2011Article
- Adapting to life at the end of the line: How Drosophila telomeric retrotransposons cope with their job.Mobile genetic elements · 2011Article
- Heterochromatin boundaries are hotspots for de novo kinetochore formation.Nature cell biology · 2011Article
- Differential maintenance of DNA sequences in telomeric and centromeric heterochromatin.Genetics · 2011Article
- An Sp185/333 gene cluster from the purple sea urchin and putative microsatellite-mediated gene diversification.BMC genomics · 2010Article
- The most frequent short sequences in non-coding DNA.Nucleic acids research · 2010Article
- Article
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8 authors.
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Abstract
The centromeric and telomeric heterochromatin of eukaryotic chromosomes is mainly composed of middle-repetitive elements, such as transposable elements and tandemly repeated DNA sequences. Because of this repetitive nature, Whole Genome Shotgun Projects have failed in sequencing these regions. We describe a novel kind of transposon-based approach for sequencing highly repetitive DNA sequences in BAC clones. The key to this strategy relies on physical mapping the precise position of the transposon insertion, which enables the correct assembly of the repeated DNA. We have applied this strategy to a clone from the centromeric region of the Y chromosome of Drosophila melanogaster. The analysis of the complete sequence of this clone has allowed us to prove that this centromeric region evolved from a telomere, possibly after a pericentric inversion of an ancestral telocentric chromosome. Our results confirm that the use of transposon-mediated sequencing, including positional mapping information, improves current finishing strategies. The strategy we describe could be a universal approach to resolving the heterochromatic regions of eukaryotic genomes.
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