ArticleThe Journal of biological chemistry2009
Cytosolic G{alpha}s acts as an intracellular messenger to increase microtubule dynamics and promote neurite outgrowth.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- The G protein-coupled estrogen receptor 1 (GPER-1) contributes to the proliferation and survival of mantle cell lymphoma cells.Haematologica · 2015Trial
- Establishing neuronal polarity: microtubule regulation during neurite initiation.Oxford open neuroscience · 2022Review
- Serotonin 5-HTCommunications biology · 2020Article
- Structural basis of Gip1 for cytosolic sequestration of G protein in wide-range chemotaxis.Nature communications · 2018Article
- Disruption of lipid-raft localized GαNeuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2018Article
- Article
- Antidepressants Accumulate in Lipid Rafts Independent of Monoamine Transporters to Modulate Redistribution of the G Protein, Gαs.The Journal of biological chemistry · 2016Article
- Activation of microtubule dynamics increases neuronal growth via the nerve growth factor (NGF)- and Gαs-mediated signaling pathways.The Journal of biological chemistry · 2015Article
- Lateral diffusion of Gαs in the plasma membrane is decreased after chronic but not acute antidepressant treatment: role of lipid raft and non-raft membrane microdomains.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2015Article
- Nerve growth factor induces neurite outgrowth of PC12 cells by promoting Gβγ-microtubule interaction.BMC neuroscience · 2014Article
- HEK-293 cells expressing the cystic fibrosis transmembrane conductance regulator (CFTR): a model for studying regulation of Cl- transport.Physiological reports · 2014Article
- Tubulin, actin and heterotrimeric G proteins: coordination of signaling and structure.Biochimica et biophysica acta · 2014Review
- Chenodeoxycholic acid stimulates Cl(-) secretion via cAMP signaling and increases cystic fibrosis transmembrane conductance regulator phosphorylation in T84 cells.American journal of physiology. Cell physiology · 2013Article
- Control of myofibroblast differentiation by microtubule dynamics through a regulated localization of mDia2.The Journal of biological chemistry · 2013Article
- Arborization of dendrites by developing neocortical neurons is dependent on primary cilia and type 3 adenylyl cyclase.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Oxidative stress contributes to lung injury and barrier dysfunction via microtubule destabilization.American journal of respiratory cell and molecular biology · 2012Article
- 5-HT7R/G12 signaling regulates neuronal morphology and function in an age-dependent manner.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- N-3 poly-unsaturated fatty acids shift estrogen signaling to inhibit human breast cancer cell growth.PloS one · 2012Article
- Non-canonical signaling and localizations of heterotrimeric G proteins.Cellular signalling · 2012Review
- Extra-long Gαs variant XLαs protein escapes activation-induced subcellular redistribution and is able to provide sustained signaling.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
It is now evident that Galpha(s) traffics into cytosol following G protein-coupled receptor activation, and alpha subunits of some heterotrimeric G-proteins, including Galpha(s) bind to tubulin in vitro. Nevertheless, many features of G-protein-microtubule interaction and possible intracellular effects of G protein alpha subunits remain unclear. In this study, several biochemical approaches demonstrated that activated Galpha(s) directly bound to tubulin and cellular microtubules, and fluorescence microscopy showed that cholera toxin-activated Galpha(s) colocalized with microtubules. The activated, GTP-bound, Galpha(s) mimicked tubulin in serving as a GTPase activator for beta-tubulin. As a result, activated Galpha(s) made microtubules more dynamic, both in vitro and in cells, decreasing the pool of insoluble microtubules without changing total cellular tubulin content. The amount of acetylated tubulin (an indicator of microtubule stability) was reduced in the presence of Galpha(s) activated by mutation. Previous studies showed that cholera toxin and cAMP analogs may stimulate neurite outgrowth in PC12 cells. However, in this study, overexpression of a constitutively activated Galpha(s) or activation of Galpha(s) with cholera toxin in protein kinase A-deficient PC12 cells promoted neurite outgrowth in a cAMP-independent manner. Thus, it is suggested that activated Galpha(s) acts as an intracellular messenger to regulate directly microtubule dynamics and promote neurite outgrowth. These data serve to link G-protein signaling with modulation of the cytoskeleton and cell morphology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.