ArticleJournal of structural biology2009
Supported double membranes.
Article in Journal of structural biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 41 citations in OpenAlex.
- Galectin-3 Binding to αThe journal of physical chemistry. B · 2022Article
- Multimodal Investigation into the Interaction of Quinacrine with Microcavity-Supported Lipid Bilayers.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Membrane anchoring facilitates colocalization of enzymes in plant cytochrome P450 redox systems.Communications biology · 2021Article
- Lipid environment of membrane proteins in cryo-EM based structural analysis.Biophysical reviews · 2018Review
- Nanostructured Lipid-based Films for Substrate Mediated Applications in Biotechnology.Advanced functional materials · 2018Article
- Supported lipid bilayer platforms to probe cell mechanobiology.Biochimica et biophysica acta. Biomembranes · 2017Review
- Article
- Multi-Stacked Supported Lipid Bilayer Micropatterning through Polymer Stencil Lift-Off.Membranes · 2015Article
- Amyloids of alpha-synuclein affect the structure and dynamics of supported lipid bilayers.Biophysical journal · 2014Article
- Biomembrane-mimicking lipid bilayer system as a mechanically tunable cell substrate.Biomaterials · 2014Article
- Characterizing the lateral friction of nanoparticles on on-chip integrated black lipid membranes.Small (Weinheim an der Bergstrasse, Germany) · 2013Article
- Model lipid bilayer with facile diffusion of lipids and integral membrane proteins.Langmuir : the ACS journal of surfaces and colloids · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Planar model membranes, like supported lipid bilayers and surface-tethered vesicles, have been proven to be useful tools for the investigation of complex biological functions in a significantly less complex membrane environment. In this study, we introduce a supported double membrane system that should be useful for studies that target biological processes in the proximity of two lipid bilayers such as the periplasm of bacteria and mitochondria or the small cleft between pre- and postsynaptic neuronal membranes. Large unilamellar vesicles (LUV) were tethered to a preformed supported bilayer by a biotin-streptavidin tether. We show from single particle tracking (SPT) experiments that these vesicle are mobile above the plane of the supported membrane. At higher concentrations, the tethered vesicles fuse to form a second continuous bilayer on top of the supported bilayer. The distance between the two bilayers was determined by fluorescence interference contrast (FLIC) microscopy to be between 16 and 24nm. The lateral diffusion of labeled lipids in the second bilayer was very similar to that in supported membranes. SPT experiments with reconstituted syntaxin-1A show that the mobility of transmembrane proteins was not improved when compared with solid supported membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.