ArticleJournal of structural biology2009
The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes.
Article in Journal of structural biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Allele-Specific Small Interfering RNA Corrects Aberrant Cellular Phenotype in Keratitis-Ichthyosis-Deafness Syndrome Keratinocytes.The Journal of investigative dermatology · 2020Article
- Concatenation of Human Connexin26 (hCx26) and Human Connexin46 (hCx46) for the Analysis of Heteromeric Gap Junction Hemichannels and Heterotypic Gap Junction Channels.International journal of molecular sciences · 2018Article
- The N-terminal Ankyrin Repeat Domain Is Not Required for Electrophile and Heat Activation of the Purified Mosquito TRPA1 Receptor.The Journal of biological chemistry · 2016Article
- Cell-free expression of a functional pore-only sodium channel.Protein expression and purification · 2015Article
- Article
- Atomic force microscopy shows connexin26 hemichannel clustering in purified membrane fragments.Biochemistry · 2014Article
- Functional analysis and regulation of purified connexin hemichannels.Frontiers in physiology · 2014Review
- Lipid-bilayer-spanning DNA nanopores with a bifunctional porphyrin anchor.Angewandte Chemie (International ed. in English) · 2013Article
- Permeation of calcium through purified connexin 26 hemichannels.The Journal of biological chemistry · 2012Article
- Green fluorescent protein changes the conductance of connexin 43 (Cx43) hemichannels reconstituted in planar lipid bilayers.The Journal of biological chemistry · 2012Article
- Electrophysiology of single and aggregate Cx43 hemichannels.PloS one · 2012Article
- Voltage-gated sodium channel (NaV) protein dissection creates a set of functional pore-only proteins.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Functional reconstitution of a voltage-gated potassium channel in giant unilamellar vesicles.PloS one · 2011Article
- CO2-dependent opening of connexin 26 and related β connexins.The Journal of physiology · 2010Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Connexin26 (Cx26) is a member of the connexin family, the building blocks for gap junction intercellular channels. These dodecameric assemblies are involved in gap junction-mediated cell-cell communication allowing the passage of ions and small molecules between two neighboring cells. Mutations in Cx26 lead to the disruption of gap junction-mediated intercellular communication with consequences such as hearing loss and skin disorders. We show here that a mutant of Cx26, M34A, forms an active hemichannel in lipid bilayer experiments. A comparison with the Cx26 wild-type is presented. Two different techniques using micro/nano-structured substrates for the formation of pore-suspending lipid membranes are used. We reconstituted the Cx26 wild-type and Cx26M34A into artificial lipid bilayers and observed single channel activity for each technique, with conductance levels of around 35, 70 and 165 pS for the wild-type. The conductance levels of Cx26M34A were found at around 45 and 70 pS.
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