ArticleBMC ophthalmology2009
CD133+ adult human retinal cells remain undifferentiated in Leukaemia Inhibitory Factor (LIF).
Article in BMC ophthalmology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 40 citations in OpenAlex.
- Boolean implication analysis of single-cell data predicts retinal cell type markers.BMC bioinformatics · 2022Article
- Longitudinal single-cell RNA-seq of hESCs-derived retinal organoids.Science China. Life sciences · 2021Article
- Bone Marrow CD133Cell transplantation · 2018Article
- Leukemia inhibitory factor increases the proliferation of human endometrial stromal cells and expression of genes related to pluripotency.International journal of reproductive biomedicine · 2017Article
- Novel Biomarker Candidates for Colorectal Cancer Metastasis: A Meta-analysis of In Vitro Studies.Cancer informatics · 2016Article
- Activated adult microglia influence retinal progenitor cell proliferation and differentiation toward recoverin-expressing neuron-like cells in a co-culture model.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2015Article
- Growth arrest and forced differentiation of human primary glioblastoma multiforme by a novel small molecule.Scientific reports · 2014Article
- Interaction of Notch and gp130 signaling in the maintenance of neural stem and progenitor cells.Cellular and molecular neurobiology · 2014Review
- Proliferation of the ciliary epithelium with retinal neuronal and photoreceptor cell differentiation in human eyes with retinal detachment and proliferative vitreoretinopathy.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2012Article
- LIF-dependent signaling: new pieces in the Lego.Stem cell reviews and reports · 2012Review
- Effective transplantation of photoreceptor precursor cells selected via cell surface antigen expression.Stem cells (Dayton, Ohio) · 2011Article
- Functional identification of cell phenotypes differentiating from mice retinal neurospheres using single cell calcium imaging.Cellular and molecular neurobiology · 2011Article
- Distinct and conserved prominin-1/CD133-positive retinal cell populations identified across species.PloS one · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCD133 is a cell surface marker of haematopoietic stem and progenitor cells. Leukaemia inhibitory factor (LIF), sustains proliferation and not differentiation of embryonic stem cells. We used CD133 to purify adult human retinal cells and aimed to determine what effect LIF had on these cultures and whether they still had the ability to generate neurospheres.
methodsRetinal cell suspensions were derived from adult human post-mortem tissue with ethical approval. With magnetic automated cell sorting (MACS) CD133+ retinal cells were enriched from post mortem adult human retina. CD133+ retinal cell phenotype was analysed by flow cytometry and cultured cells were observed for proliferative capacity, neuropshere generation and differentiation with or without LIF supplementation.
resultsWe demonstrated purification (to 95%) of CD133+ cells from adult human postmortem retina. Proliferating cells were identified through BrdU incorporation and expression of the proliferation markers Ki67 and Cyclin D1. CD133+ retinal cells differentiated whilst forming neurospheres containing appropriate lineage markers including glia, neurons and photoreceptors. LIF maintained CD133+ retinal cells in a proliferative and relatively undifferentiated state (Ki67, Cyclin D1 expression) without significant neurosphere generation. Differentiation whilst forming neurospheres was re-established on LIF withdrawal.
conclusionThese data support the evidence that CD133 expression characterises a population of cells within the resident adult human retina which have progenitor cell properties and that their turnover and differentiation is influenced by LIF. This may explain differences in retinal responses observed following disease or injury.
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