ArticleCrystal growth & design2008
LCP-FRAP Assay for Pre-Screening Membrane Proteins for in Meso Crystallization.
Article in Crystal growth & design, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Assessment of Purity, Functionality, Stability, and Lipid Composition of Cyclofos-nAChR-Detergent Complexes from Torpedo californica Using Lipid Matrix and Macroscopic Electrophysiology.The Journal of membrane biology · 2023Article
- Universal platform for the generation of thermostabilized GPCRs that crystallize in LCP.Nature protocols · 2022Review
- Article
- Protocol for crystal structure determination of the antagonist-bound human cannabinoid receptor CB2.STAR protocols · 2021Article
- Article
- Sequential purification and characterization of Torpedo californica nAChR-DC supplemented with CHS for high-resolution crystallization studies.Analytical biochemistry · 2020Article
- Changes in Membrane Protein Structural Biology.Biology · 2020Review
- Biophysical characterization dataset of native nicotinic acetylcholine receptor in lipid-like detergent complexes.Data in brief · 2020Article
- Anomalous Diffusion Characterization by Fourier Transform-FRAP with Patterned Illumination.Biophysical journal · 2020Article
- Structural insights into melatonin receptors.The FEBS journal · 2020Review
- Selection of Biophysical Methods for Characterisation of Membrane Proteins.International journal of molecular sciences · 2019Review
- Serial Femtosecond Crystallography of G Protein-Coupled Receptors.Annual review of biophysics · 2018Review
- Fluorophore Labeling, Nanodisc Reconstitution and Single-molecule Observation of a G Protein-coupled Receptor.Bio-protocol · 2017Article
- Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology.Data in brief · 2016Article
- Assessment of the functionality and stability of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology.Biochimica et biophysica acta · 2016Article
- Production of Chemokine/Chemokine Receptor Complexes for Structural Biophysical Studies.Methods in enzymology · 2016Article
- Methodological advances: the unsung heroes of the GPCR structural revolution.Nature reviews. Molecular cell biology · 2015Review
- Large-scale production and protein engineering of G protein-coupled receptors for structural studies.Frontiers in pharmacology · 2015Review
- Two classes of cholesterol binding sites for the β2AR revealed by thermostability and NMR.Biophysical journal · 2014Article
- Effects of detergent β-octylglucoside and phosphate salt solutions on phase behavior of monoolein mesophases.Biophysical journal · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Fluorescence recovery after photobleaching was used to study the diffusion of two integral membrane proteins, bacteriorhodopsin and beta2-adrenergic receptor, in lipidic cubic phase (LCP). We found that the diffusion properties within the LCP matrix strongly depend on the protein construct and applied screening conditions. Common precipitants often induce restriction on diffusion of proteins in LCP and thereby impede their chances for crystallization. A high protein mobile fraction and a fast diffusion rate correlate very well with known crystallization conditions. Using this knowledge, one can now pre-screen precipitant conditions with microgram quantities of material to rule out conditions that are not conducive to diffusion, nucleation, and crystal growth. The results of this assay will narrow membrane protein crystallization space by identifying suitable protein constructs, stabilizing compounds and precipitant conditions amenable to in meso crystallization. Crystallization pre-screening will significantly increase the chances of obtaining initial crystal hits, expediting efforts in generating high-resolution structures of challenging membrane protein targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.