ArticleGenome research2009
A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells.
Article in Genome research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 73 citations in OpenAlex.
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- A screening system for identifying interacting proteins using biomolecular fluorescence complementation and transposon gene trap.PloS one · 2021Article
- Temporal-specific roles of fragile X mental retardation protein in the development of the hindbrain auditory circuit.Development (Cambridge, England) · 2020Article
- Rapid construction of a whole-genome mutant library by combining haploid stem cells and inducible self-inactivating PiggyBac transposon.Protein & cell · 2020Article
- Review
- Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications.Stem cell reviews and reports · 2020Review
- A piggyBac-based TANGO GFP assay for high throughput screening of GPCR ligands in live cells.Cell communication and signaling : CCS · 2019Article
- Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.Nature communications · 2018Article
- Arrayed mutant haploid embryonic stem cell libraries facilitate phenotype-driven genetic screens.Nucleic acids research · 2017Article
- TrapJournal of molecular biology · 2017Article
- An optimized, broadly applicable piggyBac transposon induction system.Nucleic acids research · 2017Article
- Genome-wide barcoded transposon screen for cancer drug sensitivity in haploid mouse embryonic stem cells.Scientific data · 2017Article
- Retroviral vectors and transposons for stable gene therapy: advances, current challenges and perspectives.Journal of translational medicine · 2016Review
- Reversible Immortalization Enables Seamless Transdifferentiation of Primary Fibroblasts into Other Lineage Cells.Stem cells and development · 2016Article
- PiggyBac transposon-based polyadenylation-signal trap for genome-wide mutagenesis in mice.Scientific reports · 2016Article
- Identification and characterization of a LTR retrotransposon from the genome of Cyprinus carpio var. Jian.Genetica · 2016Article
- Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cultured mouse or human embryonic stem (ES) cells provide access to all of the genes required to elaborate the fundamental components and physiological systems of a mammalian cell. Chemical or insertional mutagenesis of Blm-deficient mouse ES cells can be used to generate genome-wide libraries of homozygous mutant ES cells, which are the substrates for conducting phenotype-driven loss-of-function genetic screens. However, the existing insertional mutation libraries are limited by incomplete genomic coverage. In this study, we have explored the use of piggyBac (PB) transposon-mediated mutagenesis to extend the genomic coverage of mutation libraries in Blm-deficient ES cells. A library composed of 14,000 individual gene-trap clones was generated and a recessive genetic screen conducted to identify cells with defects in DNA mismatch repair (MMR) genes. Independent mutations in all known genes of the pathway Msh2, Msh6, Pms2, and Mlh1 were recovered in these screens. The genomic coverage in this library confirms its utility as a new genetic resource for conducting recessive genetic screens in mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.