ArticleApplied and environmental microbiology2009
Different patterns of regulation for the copper and cadmium metallothioneins of the ectomycorrhizal fungus Hebeloma cylindrosporum.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 83 citations in OpenAlex.
- Identification of two metallothioneins in Agaricus crocodilinus reveals gene duplication and domain expansion, a pattern conserved across fungal species.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2025Article
- A Potential Involvement of Metallothionein in the Zinc Tolerance of Trichoderma harzianum: Experimental Findings.The protein journal · 2024Article
- Comparative Copper Resistance Strategies ofJournal of fungi (Basel, Switzerland) · 2022Article
- Identification and characterization of eight metallothionein genes involved in heavy metal tolerance from the ectomycorrhizal fungus Laccaria bicolor.Environmental science and pollution research international · 2022Article
- Metallothionein expression in Aspergillus exposed to environmentally relevant concentrations of heavy metals at different pH levels.Environmental science and pollution research international · 2021Article
- Mycoremediation of heavy metals: processes, mechanisms, and affecting factors.Environmental science and pollution research international · 2021Review
- In-site and Ex-site Date Palm Exposure to Heavy Metals Involved Infra-Individual Biomarkers Upregulation.Plants (Basel, Switzerland) · 2021Article
- Cd and Zn interactions and toxicity in ectomycorrhizal basidiomycetes in axenic culture.PeerJ · 2018Article
- Two PFrontiers in microbiology · 2018Article
- Article
- A comparative review towards potential of microbial cells for heavy metal removal with emphasis on biosorption and bioaccumulation.World journal of microbiology & biotechnology · 2016Review
- Model systems to unravel the molecular mechanisms of heavy metal tolerance in the ericoid mycorrhizal symbiosis.Mycorrhiza · 2016Review
- Cadmium induces cadmium-tolerant gene expression in the filamentous fungus Trichoderma harzianum.Molecular biology reports · 2015Article
- Macro and trace mineral constituents and radionuclides in mushrooms: health benefits and risks.Applied microbiology and biotechnology · 2013Review
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metallothioneins (MTs) are small cysteine-rich peptides involved in metal homeostasis and detoxification. We have characterized two MT genes, HcMT1 and HcMT2, from the ectomycorrhizal fungus Hebeloma cylindrosporum in this study. Expression of HcMT1 and HcMT2 in H. cylindrosporum under metal stress conditions was studied by competitive reverse transcription-PCR analysis. The full-length cDNAs were used to perform functional complementation in mutant strains of Saccharomyces cerevisiae. As revealed by heterologous complementation assays in yeast, HcMT1 and HcMT2 each encode a functional polypeptide capable of conferring increased tolerance against Cd and Cu, respectively. The expression levels of HcMT1 were observed to be at their maximum at 24 h, and they increased as a function of Cu concentration. HcMT2 was also induced by Cu, but the expression levels were lower than those for HcMT1. The mRNA accumulation of HcMT1 was not influenced by Cd, whereas Cd induced the transcription of HcMT2. Zn, Pb, and Ni did not affect the transcription of HcMT1 or of HcMT2. Southern blot analysis revealed that both of these genes are present as a single copy in H. cylindrosporum. While the promoters of both HcMT1 and HcMT2 contained the standard stress response elements implicated in the metal response, the numbers and varieties of potential regulatory elements were different in these promoters. These results show that ectomycorrhizal fungi encode different MTs and that each of them has a particular pattern of expression, suggesting that they play critical specific roles in improving the survival and growth of ectomycorrhizal trees in ecosystems contaminated by heavy metals.
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