ArticleNucleic acids research2009
Mutational analysis of a Dcp2-binding element reveals general enhancement of decapping by 5'-end stem-loop structures.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Conserved and divergent features of human mRNA decapping revealed by biochemical reconstitution.Nature communications · 2026Article
- Biochemical analysis of human eIF4E-DCP2 interaction: Implications for the relationship between translation initiation and decapping.PloS one · 2025Article
- Aptamer-based assay for high-throughput substrate profiling of RNA decapping enzymes.Nucleic acids research · 2024Article
- The Mimivirus L375 Nudix enzyme hydrolyzes the 5' mRNA cap.PloS one · 2021Article
- Global Profiling of Cellular Substrates of Human Dcp2.Biochemistry · 2020Article
- Mammalian Nudix proteins cleave nucleotide metabolite caps on RNAs.Nucleic acids research · 2020Article
- Characterization of the African Swine Fever Virus Decapping Enzyme during Infection.Journal of virology · 2017Article
- Interconnections between mRNA degradation and RDR-dependent siRNA production in mRNA turnover in plants.Journal of plant research · 2017Review
- New insights into decapping enzymes and selective mRNA decay.Wiley interdisciplinary reviews. RNA · 2017Review
- Dcp2 decapping protein modulates mRNA stability of the critical interferon regulatory factor (IRF) IRF-7.Molecular and cellular biology · 2012Article
- Normal and Aberrantly Capped mRNA Decapping.The Enzymes · 2012Article
- Evidence that XRN4, an Arabidopsis homolog of exoribonuclease XRN1, preferentially impacts transcripts with certain sequences or in particular functional categories.RNA (New York, N.Y.) · 2011Article
- Differential utilization of decapping enzymes in mammalian mRNA decay pathways.RNA (New York, N.Y.) · 2011Article
- Multiple mRNA decapping enzymes in mammalian cells.Molecular cell · 2010Article
- Expression of the anti-amyloidogenic secretase ADAM10 is suppressed by its 5'-untranslated region.The Journal of biological chemistry · 2010Article
- Article
- Structural and functional control of the eukaryotic mRNA decapping machinery.Biochimica et biophysica actaReview
- Regulation of mRNA decapping.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
mRNA decapping is a critical step in the control of mRNA stability and gene expression and is carried out by the Dcp2 protein. Dcp2 is an RNA-binding protein that must bind the RNA in order to recognize the cap for hydrolysis. We previously demonstrated that a 60 nucleotide (nt) element at the 5' end of the mRNA encoding Rrp41 is preferentially bound and decapped by Dcp2. Here, we demonstrate that enhanced decapping of this element is dependent on the structural integrity of its first 33 nt and not its primary sequence. The structure consists of a stem-loop positioned <10 nt from the 5' end of the mRNA. The generality of a stem-loop structure in enhanced Dcp2-mediated decapping was underscored by the identification of additional potential Dcp2 substrate mRNAs by a global analysis of human mRNAs containing a similar predicted stem-loop structure at their respective 5' end. These studies suggest a general role for 5' stem-loops in enhancing decapping activity and the utilization of this structure as a predictive tool for Dcp2 target substrates. These studies also demonstrate that Dcp2 alone in the absence of additional proteins can preferentially associate with and modulate mRNA decapping.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.