ArticleExperimental cell research2009
Myofibril assembly visualized by imaging N-RAP, alpha-actinin, and actin in living cardiomyocytes.
Article in Experimental cell research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Transcriptomic profiling of cardiac development in Bama Xiang pigs across key developmental stages.Zoological research · 2025Article
- A novel loss-of-function mutation in NRAP is associated with left ventricular non-compaction cardiomyopathy.Frontiers in cardiovascular medicine · 2023Article
- Genome-wide association study of cardiac troponin I in the general population.Human molecular genetics · 2021Article
- Disruption of Drosophila larval muscle structure and function by UNC45 knockdown.BMC molecular and cell biology · 2021Article
- Under construction: The dynamic assembly, maintenance, and degradation of the cardiac sarcomere.Journal of molecular and cellular cardiology · 2020Review
- An adapted particle swarm optimization algorithm as a model for exploring premyofibril formation.AIP advances · 2020Article
- Extracellular Matrix Structure and Composition in the Early Four-Chambered Embryonic Heart.Cells · 2020Article
- Assembly and Maintenance of Sarcomere Thin Filaments and Associated Diseases.International journal of molecular sciences · 2020Review
- Chaperones and the Proteasome System: Regulating the Construction and Demolition of Striated Muscle.International journal of molecular sciences · 2017Review
- Overview of the Muscle Cytoskeleton.Comprehensive Physiology · 2017Review
- Exome sequencing identifies variants in two genes encoding the LIM-proteins NRAP and FHL1 in an Italian patient with BAG3 myofibrillar myopathy.Journal of muscle research and cell motility · 2016Article
- Dynamic Myofibrillar Remodeling in Live Cardiomyocytes under Static Stretch.Scientific reports · 2016Article
- Pacemaker-induced transient asynchrony suppresses heart failure progression.Science translational medicine · 2015Article
- Jasplakinolide reduces actin and tropomyosin dynamics during myofibrillogenesis.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Xin proteins and intercalated disc maturation, signaling and diseases.Frontiers in bioscience (Landmark edition) · 2012Review
- At the Start of the Sarcomere: A Previously Unrecognized Role for Myosin Chaperones and Associated Proteins during Early Myofibrillogenesis.Biochemistry research international · 2012Article
- Article
- Cardiac-specific NRAP overexpression causes right ventricular dysfunction in mice.Experimental cell research · 2011Article
- Scaffolds and chaperones in myofibril assembly: putting the striations in striated muscle.Biophysical reviews · 2011Article
- The Nebulin family: an actin support group.Trends in cell biology · 2011Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
N-RAP is a striated muscle-specific scaffolding protein that organizes alpha-actinin and actin into symmetrical I-Z-I structures in developing myofibrils. Here we determined the order of events during myofibril assembly through time-lapse confocal microscopy of cultured embryonic chick cardiomyocytes coexpressing fluorescently tagged N-RAP and either alpha-actinin or actin. During de novo myofibril assembly, N-RAP assembled in fibrillar structures within the cell, with dots of alpha-actinin subsequently organizing along these structures. The initial fibrillar structures were reminiscent of actin fibrils, and coassembly of N-RAP and actin into newly formed fibrils supported this. The alpha-actinin dots subsequently broadened to Z-lines that were wider than the underlying N-RAP fibril, and N-RAP fluorescence intensity decreased. FRAP experiments showed that most of the alpha-actinin dynamically exchanged during all stages of myofibril assembly. In contrast, less than 20% of the N-RAP in premyofibrils was exchanged during 10-20 min after photobleaching, but this value increased to 70% during myofibril maturation. The results show that N-RAP assembles into an actin containing scaffold before alpha-actinin recruitment; that the N-RAP scaffold is much more stable than the assembling structural components; that N-RAP dynamics increase as assembly progresses; and that N-RAP leaves the structure after assembly is complete.
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