ArticleBiochimica et biophysica acta2009
Thermal and chemical unfolding and refolding of a eukaryotic sodium channel.
Article in Biochimica et biophysica acta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
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- Macromolecular rate theory explains the temperature dependence of membrane conductance kinetics.Biophysical journal · 2023Article
- Probing the physiological roles of the extracellular loops of chitoporin from Vibrio campbellii.Biophysical journal · 2021Article
- Thermal melt circular dichroism spectroscopic studies for identifying stabilising amphipathic molecules for the voltage-gated sodium channel NavMs.Biopolymers · 2018Article
- Three-dimensional Modelling of the Voltage-gated Sodium Ion Channel from Anopheles gambiae Reveals Spatial Clustering of Evolutionarily Conserved Acidic Residues at the Extracellular Sites.Current neuropharmacology · 2017Article
- Attenuation of Phosphorylation-dependent Activation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Disease-causing Mutations at the Transmission Interface.The Journal of biological chemistry · 2017Article
- Benchmarking the stability of human detergent-solubilised voltage-gated sodium channels for structural studies using eel as a reference.Biochimica et biophysica acta · 2015Article
- Structural characteristics of thermostable immunogenic outer membrane protein from Salmonella enterica serovar Typhi.Applied microbiology and biotechnology · 2014Article
- Studying the Stability of S-Layer Protein of Lactobacillus Acidophilus ATCC 4356 in Simulated Gastrointestinal Fluids Using SDS-PAGE and Circular Dichroism.Iranian journal of pharmaceutical research : IJPR · 2013Article
- In vitro folding of KvAP, a voltage-gated K+ channel.Biochemistry · 2011Article
- NaChBac: the long lost sodium channel ancestor.Biochemistry · 2011Article
- Voltage-gated sodium channel (NaV) protein dissection creates a set of functional pore-only proteins.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Negative staining and cryo-negative staining of macromolecules and viruses for TEM.Micron (Oxford, England : 1993) · 2011Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Voltage-gated sodium channels are dynamic membrane proteins essential for signaling in nervous and muscular systems. They undergo substantial conformational changes associated with the closed, open and inactivated states. However, little information is available regarding their conformational stability. In this study circular dichroism spectroscopy was used to investigate the changes in secondary structure accompanying chemical and thermal denaturation of detergent-solubilised sodium channels isolated from Electrophorus electricus electroplax. The proteins appear to be remarkably resistant to either type of treatment, with "denatured" channels, retaining significant helical secondary structure even at 77 degrees C or in 10% SDS. Further retention of helical secondary structure at high temperature was observed in the presence of the channel-blocking tetrodotoxin. It was possible to refold the thermally-denatured (but not chemically-denatured) channels in vitro. The correctly refolded channels were capable of undergoing the toxin-induced conformational change indicative of ligand binding. In addition, flux measurements in liposomes showed that the thermally-denatured (but not chemically-denatured) proteins were able to re-adopt native, active conformations. These studies suggest that whilst sodium channels must be sufficiently flexible to undergo major conformational changes during their functional cycle, the proteins are highly resistant to unfolding, a feature that is important for maintaining structural integrity during dynamic processes.
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