ArticleAnalytical biochemistry2009
Quantitative capillary electrophoresis determination of oversulfated chondroitin sulfate as a contaminant in heparin preparations.
Article in Analytical biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 66 citations in OpenAlex.
- Tools for the Quality Control of Pharmaceutical Heparin.Medicina (Kaunas, Lithuania) · 2019Review
- Implementation of infrared and Raman modalities for glycosaminoglycan characterization in complex systems.Glycoconjugate journal · 2017Review
- Differential interactions of conjugated polymer nanoparticles with glycosaminoglycans in synthetic urine.Chemical communications (Cambridge, England) · 2015Article
- Analysis of glycosaminoglycan-derived disaccharides by capillary electrophoresis using laser-induced fluorescence detection.Analytical biochemistry · 2012Article
- Electrophoresis for the analysis of heparin purity and quality.Electrophoresis · 2012Review
- Cell-Based Microscale Isolation of Glycoaminoglycans for Glycomics Study.Journal of carbohydrate chemistry · 2012Article
- Disaccharide analysis of glycosaminoglycan mixtures by ultra-high-performance liquid chromatography-mass spectrometry.Journal of chromatography. A · 2012Article
- Linear polyalkylamines as fingerprinting agents in capillary electrophoresis of low-molecular-weight heparins and glycosaminoglycans.Electrophoresis · 2011Article
- Competitive inhibition of heparinase by persulfonated glycosaminoglycans: a tool to detect heparin contamination.Analytical chemistry · 2011Article
- Ultra-performance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis.Analytical biochemistry · 2011Article
- Hyphenated techniques for the analysis of heparin and heparan sulfate.Analytical and bioanalytical chemistry · 2011Review
- Mass balance analysis of contaminated heparin product.Analytical biochemistry · 2011Article
- Analysis and characterization of heparin impurities.Analytical and bioanalytical chemistry · 2011Review
- Oversulfated chondroitin sulfate is not the sole contaminant in heparin.Nature biotechnology · 2010Article
- Orthogonal analytical approaches to detect potential contaminants in heparin.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
A simple, accurate, and robust quantitative capillary electrophoresis (CE) method for the determination of oversulfated chondroitin sulfate (OSCS) as a contaminant in heparin (Hep) preparations is described. After degradation of the polysaccharides by acidic hydrolysis, the hexosamines produced (i.e., GlcN from Hep and GalN from OSCS) were derivatized with anthranilic acid (AA) and separated by means of CE in approximately 10 min with high sensitivity detection at 214 nm (limit of detection [LOD] of approximately 200 pg). Furthermore, AA-derivatized GlcN and GalN showed quite similar molar absorptivity, allowing direct and simple quantification of OSCS in Hep samples. Moreover, a preliminary step of specific enzymatic treatment by using chondroitin ABC lyase may be applied for the specific elimination of interference in the analysis due to the possible presence in Hep samples of natural chondroitin sulfate and dermatan sulfate impurities, making this analytical approach highly specific for OSCS contamination given that chondroitin ABC lyase is unable to act on this semisynthetic polymer. The CE method was validated for specificity, linearity, accuracy, precision, LOD, and limit of quantification (LOQ). Due to the very high sensitivity of CE, as little as 1% OSCS contaminant in Hep sample could be detected and quantified. Finally, a contaminated raw Hep sample was found to contain 38.9% OSCS, whereas a formulated contaminated Hep was calculated to have 39.7% OSCS.
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Registered trials
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