Evidence map›Paper›PMID 19230968›Full record

ArticlePlacenta2009

Differential expression of fibulin family proteins in the para-cervical weak zone and other areas of human fetal membranes.

R M Moore, R W Redline, D Kumar, B M Mercer, J M Mansour, E Yohannes, J B Novak, M R Chance, J J Moore

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Placenta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
  2. Review
  3. Observational
  4. Review
  5. Article
  6. Extracellular matrix dynamics and fetal membrane rupture.Reproductive sciences (Thousand Oaks, Calif.) · 2013
    Review
  7. Article
  8. Fibulin-5 expression in the human placenta.Histochemistry and cell biology · 2011
    Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

R M MooreDepartment of Pediatrics, Case Western Reserve University, Cleveland, OH, United States.
R W Redline
D Kumar
B M Mercer
J M Mansour
E Yohannes
J B Novak
M R Chance
J J Moore
Case Western Reserve University · US

Funding

Stretch and Apoptosis Cause Fetal Membrane RuptureR01HD048476 · NICHD · CASE WESTERN RESERVE UNIVERSITY · PI MOORE, JOHN J · 2005 to 2009
$1.6M
NICHD NIH HHS R01 HD048476
6 · The paper itself

Abstract

objectiveHuman fetal membranes (FM) at term have been shown to contain a weak zone in the region overlying the cervix which exhibits characteristics of increased collagen remodeling and apoptosis. It has been hypothesized that the FM rupture initiation site is within this weak zone. Although the FM weak zone has been partially characterized, it is unclear what structural differences in the extracellular matrix result in its decreased rupture strength. A screen for differentially expressed proteins in the amnion of the weak zone versus other FM areas demonstrated that fibulin 1 was decreased. We investigated potential regional differences in all fibulin protein family members.

methodsFM fibulins were localized by immunohistochemistry. Detected fibulins were screened by Western blot for differences in abundance in the amnion of the weak zone versus non-weak zone FM regions. Amnion epithelial and mesenchymal cells were also screened for fibulin production.

resultsFibulins 1 and 5 were detected in the cytoplasm of and in a pericellular pattern surrounding all FM cells, and in a dense extracellular pattern in the amniotic compact zone. Fibulin 3 was detected within the cytoplasm of amnion epithelial and chorion trophoblast cells. Fibulins 2 and 4 were not detected. Fibulins 1, 3 and 5 demonstrated decreased abundance of 33%, 63% and 58% (all P<0.01) in amnion of the weak zone relative to other FM regions. Amnion cells produced all three detected fibulins. Furthermore, TNF inhibited amnion cell fibulin production in a dose dependent manner.

conclusionFibulins 1, 3 and 5 were localized coincident with major microfibrillar networks in amnion. Each showed decreased abundance in the amnion component of the FM weak zone. Amnion epithelial and mesenchymal cells produced all three fibulins and their abundance was inhibited by TNF. We speculate that the amnion microfibrillar layer undergoes significant remodeling with the development of the FM weak zone.

Indexed as

AmnionBiomechanical PhenomenaBlotting, WesternCalcium-Binding ProteinsCells, CulturedCervix UteriDown-RegulationExtracellular Matrix ProteinsExtraembryonic MembranesFemaleHumansImmunohistochemistryIn Vitro TechniquesPregnancyProtein Array AnalysisProteomeCalcium-Binding ProteinsEFEMP1 protein, humanExtracellular Matrix ProteinsFBLN5 protein, humanfibulinProteome

Identifiers

PMID19230968
PMCPMC2693720
OpenAlexW1985593083

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.