ArticleBiosensors & bioelectronics2009
Use of electric cell-substrate impedance sensing to assess in vitro cytotoxicity.
Article in Biosensors & bioelectronics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed.
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- Label-free in vitro assays predict the potency of anti-disialoganglioside chimeric antigen receptor T-cell products.Cytotherapy · 2023Article
- High-accuracy gastric cancer cell viability evaluation based on multi-impedance spectrum characteristics.Heliyon · 2023Article
- Extracellularly Detectable Electrochemical Signals of Living Cells Originate from Metabolic Reactions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Odorant Binding Causes Cytoskeletal Rearrangement, Leading to Detectable Changes in Endothelial and Epithelial Barrier Function and Micromotion.Biosensors · 2023Article
- Antiproliferative Properties of Triterpenoids by ECIS Method-A New Promising Approach in Anticancer Studies?Molecules (Basel, Switzerland) · 2022Article
- Real-Time Monitoring the Cytotoxic Effect of Andrographolide on Human Oral Epidermoid Carcinoma Cells.Biosensors · 2022Article
- ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates.Biosensors · 2022Article
- Online Measurement System for Dynamic Flow Bioreactors to Study Barrier Integrity of hiPSC-Based Blood-Brain Barrier In Vitro Models.Bioengineering (Basel, Switzerland) · 2022Article
- Can ECIS Biosensor Technology Be Used to Measure the Cellular Responses of Glioblastoma Stem Cells?Biosensors · 2021Article
- Application of impedance measurement to investigate in vitro inhalation toxicity of bacteria.Journal of occupational medicine and toxicology (London, England) · 2021Article
- Detecting Effects of Low Levels of FCCP on Stem Cell Micromotion and Wound-Healing Migration by Time-Series Capacitance Measurement.Sensors (Basel, Switzerland) · 2021Article
- Differences of the tumour cell glycocalyx affect binding of capsaicin-loaded chitosan nanocapsules.Scientific reports · 2020Article
- Potential antitumor activity of digitoxin and user-designed analog administered to human lung cancer cells.Biochimica et biophysica acta. General subjects · 2020Article
- Use of Discrete Wavelet Transform to Assess Impedance Fluctuations Obtained from Cellular Micromotion.Sensors (Basel, Switzerland) · 2020Article
- Polymer-Based Functional Cantilevers Integrated with Interdigitated Electrode Arrays-A Novel Platform for Cardiac Sensing.Micromachines · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
In vitro assessment of cytotoxicity based on electrochemical impedance spectroscopy (EIS) needs more quantitative methods to analyze the alteration of cell morphology and motility, and hence the potential risk to human health. Here, we applied electric cell-substrate impedance sensing (ECIS) to evaluate dose-dependent responses of human umbilical vein endothelial cells exposed to cytochalasin B. To detect subtle changes in cell morphology, the frequency-dependent impedance data of the cell monolayer were measured and analyzed with a theoretical cell-electrode model. To detect the alternation of cell micromotion in response to cytochalasin B challenge, time-series impedance fluctuations of cell-covered electrodes were monitored and the values of power spectrum, variance, and variance of the increments were calculated to verify the difference. While a dose-dependent relationship was generally observed from the overall resistance of the cell monolayer, the analysis of frequency-dependent impedance and impedance fluctuations distinguished cytochalasin B levels as low as 0.1 microM. Our results show that cytochalasin B causes a decrease of junctional resistance between cells, an increase of membrane capacitance, and the reduction in micromotion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.