ArticleNature protocols2009
A robust plant RNA isolation method suitable for Affymetrix GeneChip analysis and quantitative real-time RT-PCR.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 70 citations in OpenAlex.
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- A modified approach for high-quality RNA extraction of spore-forming Bacillus subtilis at varied physiological stages.Molecular biology reports · 2021Article
- A universal method for high-quality RNA extraction from plant tissues rich in starch, proteins and fiber.Scientific reports · 2020Article
- LC-MS/MS Profiling of Post-Transcriptional Modifications in Ginseng tRNA Purified by a Polysaccharase-Aided Extraction Method.Biomolecules · 2020Article
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- Ectopic Expression ofInternational journal of molecular sciences · 2020Article
- Metabolite and transcript profiling of Guinea grass (Panicum maximum Jacq) response to elevated [COMetabolomics : Official journal of the Metabolomic Society · 2019Article
- QRREM method for the isolation of high-quality RNA from the complex matrices of coconut.Bioscience reports · 2019Article
- Field-grown soybean transcriptome shows diurnal patterns in photosynthesis-related processes.Plant direct · 2018Article
- Recent amplification of Osr4 LTR-retrotransposon caused ricePlant diversity · 2017Article
- Effects of High Glucose on Cell Viability and Differentiation in Primary Cultured Schwann Cells: Potential Role of ERK Signaling Pathway.Neurochemical research · 2016Article
- Transcriptomic Analysis Using Olive Varieties and Breeding Progenies Identifies Candidate Genes Involved in Plant Architecture.Frontiers in plant science · 2016Article
- A comparative analysis of transcriptomic, biochemical, and physiological responses to elevated ozone identifies species-specific mechanisms of resilience in legume crops.Journal of experimental botany · 2015Article
- Functional Characterization of Phalaenopsis aphrodite Flowering Genes PaFT1 and PaFD.PloS one · 2015Article
- A simple method for RNA isolation from various tissues of the treeBiotechnology, biotechnological equipment · 2014Article
- CKC: isolation of nucleic acids from a diversity of plants using CTAB and silica columns.Molecular biotechnology · 2013Article
- RNA-Seq analysis of Cocos nucifera: transcriptome sequencing and de novo assembly for subsequent functional genomics approaches.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microarray analysis and quantitative real-time RT-PCR are the major high-throughput techniques that are used to study transcript profiles. One of the major limitations in these technologies is the isolation of large quantities of highly pure RNA from plant tissues rich in complex polysaccharides, polyphenolics and waxes. Any contamination of the isolated RNA affects the downstream applications and requires extra cleaning procedures that result in a reduced RNA yield, especially the low molecular weight molecules. The protocol presented here is suitable for isolating high yield and clean total RNA from field-grown plants. Unlike current methods, such as LiCl and TRIZOL, with this new method, the isolated RNA can be used directly for Affymetrix GeneChip labeling or real-time RT-PCR without further purification. This fast and simple protocol provides ready-to-use RNA within 4-5 h after sampling. Additionally, the protocol described here maintains the isolation of small RNA molecules, making it an ideal choice for plant RNA preparation prior to high-throughput sequencing methods to study gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.