ArticleMolecular endocrinology (Baltimore, Md.)2009
Regulation of gap junctions in porcine cumulus-oocyte complexes: contributions of granulosa cell contact, gonadotropins, and lipid rafts.
Article in Molecular endocrinology (Baltimore, Md.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 116 citations in OpenAlex.
- Changes in Ras and Ras-Associated GTPases During Maturation of Porcine Cumulus Cells and Oocytes.Animals : an open access journal from MDPI · 2026Article
- Effect of Low-Temperature Plasma on Porcine Oocytes In Vitro Primary Culture.Antioxidants (Basel, Switzerland) · 2026Article
- Unraveling the Molecular Landscape of Follicular Fluid: A Comparative Proteomic Study Between Bali and Brahman Cattle.Veterinary medicine international · 2026Article
- TRIB3 regulates Cx43 expression in human granulosa cells via the Wnt/β-catenin signaling pathway: an in vitro study.Journal of ovarian research · 2025Article
- Single-cell and spatiotemporal profile of ovulation in the mouse ovary.PLoS biology · 2025Article
- The follicle-stimulating hormone triggers rapid changes in mitochondrial structure and function in porcine cumulus cells.Scientific reports · 2024Article
- Cyclic AMP mediates ovine cumulus-oocyte gap junctional function via balancing connexin 43 expression and phosphorylation.Endocrine connections · 2023Article
- BPA interferes with granulosa cell development and oocyte meiosis in mouse preantral follicles.Experimental biology and medicine (Maywood, N.J.) · 2023Article
- Lab partners: oocytes, embryos and company. A personal view on aspects of oocyte maturation and the development of monozygotic twins.Animal reproduction · 2023Article
- A Lack of GD3 Synthase Leads to Impaired Renal Expression of Connexins and Pannexin1 inInternational journal of molecular sciences · 2022Article
- The Molecular Quality and Mitochondrial Activity of Porcine Cumulus-Oocyte Complexes Are Affected by Their Exposure to Three Endocrine-Active Compounds under 3D In Vitro Maturation Conditions.International journal of molecular sciences · 2022Article
- The Function of Cumulus Cells in Oocyte Growth and Maturation and in Subsequent Ovulation and Fertilization.Cells · 2021Review
- Pannexins and Connexins: Their Relevance for Oocyte Developmental Competence.International journal of molecular sciences · 2021Review
- Effect of porcine immature oocyte vitrification on oocyte-cumulus cell gap junctional intercellular communication.Porcine health management · 2020Article
- Luteinizing Hormone Action in Human Oocyte Maturation and Quality: Signaling Pathways, Regulation, and Clinical Impact.Reproductive sciences (Thousand Oaks, Calif.) · 2020Review
- Resolvin E1 in Follicular Fluid Acts as a Potential Biomarker and Improves Oocyte Developmental Competence by Optimizing Cumulus Cells.Frontiers in endocrinology · 2020Article
- Removal of cumulus cells around 20 h after the start of in vitro maturation improves the meiotic competence of porcine oocytes via reduction in cAMP and cGMP levels.The Journal of reproduction and development · 2019Article
- Functional role of Forskolin and PD166285 in the development of denuded mouse oocytes.Asian-Australasian journal of animal sciences · 2018Article
- Article
- Cyclic guanosine monophosphate does not inhibit gonadotropin-induced activation of mitogen-activated protein kinase 3/1 in pig cumulus-oocyte complexes.Reproductive biology and endocrinology : RB&E · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gap-junctional communication (GJC) plays a central role in oocyte growth. However, little is known about the regulation of connexin 43 (Cx43)-based gap-junction channels in cumulus-oocyte complexes (COCs) during in vitro maturation. We show that rupture of COCs from mural granulosa cells up-regulates Cx43-mediated GJC and that gonadotropins signal GJC breakdown by recruiting Cx43 to lipid rafts when oocyte meiosis resumes. Oocyte calcein uptake through gap junctions increases during early in vitro oocyte maturation and remains high until 18 h, when it falls simultaneously with the oocyte germinal vesicle breakdown. Immunodetection of Cx43 and fluorescence recovery after photobleaching assays revealed that the increase of GJC is independent of gonadotropins but requires RNA transcription, RNA polyadenylation, and translation. GJC rupture, in contrast, is achieved by a gonadotropin-dependent mechanism involving recruitment of Cx43 to clustered lipid rafts. These results show that GJC up-regulation in COCs in in vitro culture is independent of gonadotropins and transcriptionally regulated. However, GJC breakdown is gonadotropin dependent and mediated by the clustering of Cx43 in lipid raft microdomains. In conclusion, this study supports a functional role of lipid raft clustering of Cx43 in GJC breakdown in the COCs during in vitro maturation.
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