ArticleGenome research2009
Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system.
Article in Genome research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 78 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
78 citing papers in PubMed, 147 citations in OpenAlex.
- The regulation of AsfR on tmRNA expression mediates bacterial motility and virulence inVirulence · 2026Article
- Quorum Sensing Regulator CinR Directly Activates the Catalase-Peroxidase GeneAntioxidants (Basel, Switzerland) · 2026Article
- Regulation of iron homeostasis by Fur and atypical response regulator SsoR via derepressor-inhibitor oscillation inApplied and environmental microbiology · 2025Article
- Rewiring of cell morphology by Small protein B enhances stress tolerance and colonization in Aeromonas veronii under adverse conditions.Nucleic acids research · 2025Article
- A novel two-component system contributing the catabolism of c-di-GMP influences virulence inFrontiers in microbiology · 2025Article
- ArgR regulates motility and virulence through positive control of flagellar genes and inhibition of diguanylate cyclase expression in Aeromonas veronii.Communications biology · 2024Article
- Copper sensing transcription factor ArsR2 regulates VjbR to sustain virulence inEmerging microbes & infections · 2024Article
- D-Xylose Blocks the Broad Negative Regulation of XylR on Lipid Metabolism and Affects Multiple Physiological Characteristics in Mycobacteria.International journal of molecular sciences · 2023Article
- An intricate regulation of WblA controlling production of silent tylosin analogues and abolishment of expressible nikkomycin.Science China. Life sciences · 2023Article
- Article
- Article
- Diguanylate Cyclase and Phosphodiesterase Interact To Maintain the Specificity of Cyclic di-GMP Signaling in the Regulation of Antibiotic Synthesis in Lysobacter enzymogenes.Applied and environmental microbiology · 2022Article
- Peptide Aptamer PA3 Attenuates the Viability ofFrontiers in microbiology · 2022Article
- The predatory soil bacterium Lysobacter reprograms quorum sensing system to regulate antifungal antibiotic production in a cyclic-di-GMP-independent manner.Communications biology · 2021Article
- Label-Free Comparative Proteomics of Differentially ExpressedPathogens (Basel, Switzerland) · 2021Article
- Combining transposon mutagenesis and reporter genes to identify novel regulators of the topA promoter in Streptomyces.Microbial cell factories · 2021Article
- Structural visualization of transcription activated by a multidrug-sensing MerR family regulator.Nature communications · 2021Article
- Article
- Coordinated control of the type IV pili and c-di-GMP-dependent antifungal antibiotic production in Lysobacter by the response regulator PilR.Molecular plant pathology · 2021Article
- Elucidation of the Pathogenicity-Associated Regulatory Network in Xanthomonas oryzae pv. oryzae.mSystems · 2021Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequence-specific DNA-binding transcription factors have widespread biological significance in the regulation of gene expression. However, in lower prokaryotes and eukaryotic metazoans, it is usually difficult to find transcription regulatory factors that recognize specific target promoters. To address this, we have developed in this study a new bacterial one-hybrid reporter vector system that provides a convenient and rapid strategy to determine the specific interaction between target DNA sequences and their transcription factors. Using this system, we have successfully determined the DNA-binding specificity of the transcription regulator Rv3133c to a previously reported promoter region of the gene Rv2031 in Mycobacterium tuberculosis. In addition, we have tested more than 20 promoter regions of M. tuberculosis genes using this approach to determine if they interact with approximately 150 putative regulatory proteins. A variety of transcription factors are found to participate in the regulation of stress response and fatty acid metabolism, both of which comprise the core of in vivo-induced genes when M. tuberculosis invades macrophages. Interestingly, among the many new discovered potential transcription factors, the WhiB-like transcriptional factor WhiB3 was identified for the first time to bind with the promoter sequences of most in vivo-induced genes. Therefore, this study offers important data in the dissection of the transcription regulations in M. tuberculosis, and the strategy should be applicable in the study of DNA-binding factors in a wide range of biological organisms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.