ArticleJournal of proteome research2009
S-layer, surface-accessible, and concanavalin A binding proteins of Methanosarcina acetivorans and Methanosarcina mazei.
Article in Journal of proteome research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Cell surface differences within the genusJournal of bacteriology · 2025Review
- Composition and in situ structure of theScience advances · 2024Article
- Genome reduction in novel, obligately methyl-reducing Methanosarcinales isolated from arthropod guts (Methanolapillus gen. nov. and Methanimicrococcus).FEMS microbiology ecology · 2024Article
- Comprehensive glycoproteomics shines new light on the complexity and extent of glycosylation in archaea.PLoS biology · 2021Article
- Genomics of Clostridium taeniosporum, an organism which forms endospores with ribbon-like appendages.PloS one · 2018Article
- Emerging facets of prokaryotic glycosylation.FEMS microbiology reviews · 2017Review
- Archaeal S-Layers: Overview and Current State of the Art.Frontiers in microbiology · 2017Review
- Membrane biofilm development improves COD removal in anaerobic membrane bioreactor wastewater treatment.Microbial biotechnology · 2015Article
- Mining proteomic data to expose protein modifications in Methanosarcina mazei strain Gö1.Frontiers in microbiology · 2015Article
- The complete genome sequence of the rumen methanogen Methanosarcina barkeri CM1.Standards in genomic sciences · 2015Article
- N-linked glycosylation in Archaea: a structural, functional, and genetic analysis.Microbiology and molecular biology reviews : MMBR · 2014Review
- Microbial ecology of anaerobic digesters: the key players of anaerobiosis.TheScientificWorldJournal · 2014Review
- Effects of antimicrobial peptides on methanogenic archaea.Antimicrobial agents and chemotherapy · 2012Article
- Structure of the surface layer of the methanogenic archaean Methanosarcina acetivorans.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Identification of the major expressed S-layer and cell surface-layer-related proteins in the model methanogenic archaea: Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A.Archaea (Vancouver, B.C.) · 2012Article
- Archaeosortases and exosortases are widely distributed systems linking membrane transit with posttranslational modification.Journal of bacteriology · 2012Article
- The S-layer glycoprotein of the crenarchaeote Sulfolobus acidocaldarius is glycosylated at multiple sites with chitobiose-linked N-glycans.Archaea (Vancouver, B.C.) · 2010Article
- S-layer glycoproteins and flagellins: reporters of archaeal posttranslational modifications.Archaea (Vancouver, B.C.) · 2010Review
- Shaping the archaeal cell envelope.Archaea (Vancouver, B.C.) · 2010Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
The outermost cell envelope structure of many archaea and bacteria contains a proteinaceous lattice termed the surface layer or S-layer. It is typically composed of only one or two abundant, often posttranslationally modified proteins that self-assemble to form the highly organized arrays. Surprisingly, over 100 proteins were annotated to be S-layer components in the archaeal species Methanosarcina acetivorans C2A and Methanosarcina mazei Gö1, reflecting limitations of current predictions. An in vivo biotinylation methodology was devised to affinity tag surface-exposed proteins while overcoming unique challenges in working with these fragile organisms. Cells were adapted to growth under N2 fixing conditions, thus, minimizing free amines reactive to the NHS-label, and high pH media compatible with the acylation chemistry was used. A 3-phase separation procedure was employed to isolate intact, labeled cells from lysed-cell derived proteins. Streptavidin affinity enrichment followed by stringent wash conditions removed nonspecifically bound proteins. This methodology revealed S-layer proteins in M. acetivorans C2A and M. mazei Gö1 to be MA0829 and MM1976, respectively. Each was demonstrated to exist as multiple glycosylated forms using SDS-PAGE coupled with glycoprotein-specific staining, and by interaction with the lectin, Concanavalin A. A number of additional surface-exposed proteins and glycoproteins were identified and included all three subunits of the thermosome: the latter suggests that the chaperonin complex is both surface- and cytoplasmically localized. This approach provides an alternative strategy to study surface proteins in the archaea.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.