Evidence map›Paper›PMID 19225541›Full record

ArticleThe Journal of investigative dermatology2009

Wounding-induced synthesis of hyaluronic acid in organotypic epidermal cultures requires the release of heparin-binding egf and activation of the EGFR.

James Monslow, Nobuyuki Sato, Judith A Mack, Edward V Maytin

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of investigative dermatology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 49 citations in OpenAlex.

  1. Review
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  10. Modeling vitamin D actions in triple negative/basal-like breast cancer.The Journal of steroid biochemistry and molecular biology · 2014
    Review
  11. Article
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  15. Regenerative potential of glycosaminoglycans for skin and bone.Journal of molecular medicine (Berlin, Germany) · 2012
    Review
  16. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

James MonslowDepartment of Biomedical Engineering, Cleveland Clinic, Cleveland, Ohio 44195, USA.
Nobuyuki Sato
Judith A Mack
Edward V Maytin
Cleveland Clinic · US

Funding

HYALURONAN AND EPIDERMAL DIFFERENTIATIONR01AR049249 · NIAMS · CLEVELAND CLINIC LERNER COM-CWRU · PI MAYTIN, EDWARD V. · 2004 to 2007
$1.1M
NIAMS NIH HHS R01 AR049249
6 · The paper itself

Abstract

Hyaluronic acid (HA), a glycosaminoglycan located between keratinocytes in the epidermis, accumulates dramatically following skin wounding. To study inductive mechanisms, a rat keratinocyte organotypic culture model that faithfully mimics HA metabolism was used. Organotypic cultures were needle-punctured 100 times, incubated for up to 24 hours, and HA analyzed by histochemical and biochemical methods. Within 15 minutes post-injury, HA levels had elevated two-fold, increasing to four-fold by 24 hours. HA elevations far from the site of injury suggested the possible involvement of a soluble HA-inductive factor. Media transfer experiments (from wounded cultures to unwounded cultures) confirmed the existence of a soluble factor. From earlier evidence, we hypothesized that an EGF-like growth factor might be responsible. This was confirmed as follows: (1) EGFR kinase inhibitor (AG1478) completely prevented wounding-induced HA accumulation. (2) Rapid tyrosine-phosphorylation of EGFR correlated well with the onset of increased HA synthesis. (3) A neutralizing antibody that recognizes heparin binding EGF-like growth factor (HB-EGF) blocked wounding-induced HA synthesis by > or =50%. (4) Western analyses showed that release of activated HB-EGF (but neither amphiregulin nor EGF) occured after wounding. In summary, rapid HA accumulation after epidermal wounding occurs through a mechanism requiring cleavage of HB-EGF and activation of EGFR signaling.

Indexed as

AnimalsCell LineDogsEpidermisErbB ReceptorsHeparin-binding EGF-like Growth FactorHumansHyaluronic AcidIntercellular Signaling Peptides and ProteinsMiceMice, Inbred C57BLRatsSignal TransductionWound HealingWounds and InjuriesErbB ReceptorsHBEGF protein, humanHbegf protein, mouseHbegf protein, ratHeparin-binding EGF-like Growth FactorHyaluronic AcidIntercellular Signaling Peptides and Proteins

Identifiers

PMID19225541
PMCPMC2811374
OpenAlexW2049460207

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.