ArticleThe Journal of molecular diagnostics : JMD2009
Multi-Site PCR-based CMV viral load assessment-assays demonstrate linearity and precision, but lack numeric standardization: a report of the association for molecular pathology.
Article in The Journal of molecular diagnostics : JMD, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Analytical Performance and Inter-Method Agreement of a Laboratory-Developed CMV qPCR Assay in Clinical Plasma Samples.Microorganisms · 2026Article
- Forty Years of Molecular Diagnostics for Infectious Diseases.Journal of clinical microbiology · 2022Review
- Implementation and Validation of the Roche Light Cycler 480 96-Well Plate Platform as a Real-Time PCR Assay for the Quantitative Detection of Cytomegalovirus (CMV) in Clinical Specimens Using the Luminex MultiCode ASRs System.Medical sciences (Basel, Switzerland) · 2020Article
- Automated Nucleic Acid Extraction Systems for Detecting Cytomegalovirus and Epstein-Barr Virus Using Real-Time PCR: A Comparison Study Between the QIAsymphony RGQ and QIAcube Systems.Annals of laboratory medicine · 2017Article
- Commutability of the First World Health Organization International Standard for Human Cytomegalovirus.Journal of clinical microbiology · 2015Article
- Monitoring of cytomegalovirus viral loads by two molecular assays in whole-blood and plasma samples from hematopoietic stem cell transplant recipients.Journal of clinical microbiology · 2015Article
- Clinical Applications of Quantitative Real-Time PCR in Virology.Methods in microbiology · 2015Article
- Clinical utility of droplet digital PCR for human cytomegalovirus.Journal of clinical microbiology · 2014Article
- Multicenter evaluation of a commercial cytomegalovirus quantitative standard: effects of commutability on interlaboratory concordance.Journal of clinical microbiology · 2013Article
- Clinical utility of viral load in management of cytomegalovirus infection after solid organ transplantation.Clinical microbiology reviews · 2013Review
- Fully automated quantification of cytomegalovirus (CMV) in whole blood with the new sensitive Abbott RealTime CMV assay in the era of the CMV international standard.Journal of clinical microbiology · 2013Article
- Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus.Journal of clinical microbiology · 2013Article
- Clinical significance of low cytomegalovirus DNA levels in human plasma.Journal of clinical microbiology · 2012Article
- Factors contributing to variability of quantitative viral PCR results in proficiency testing samples: a multivariate analysis.Journal of clinical microbiology · 2012Article
- Viral infection in renal transplant recipients.TheScientificWorldJournal · 2012Review
- [Cytomegalovirus. Pathological-anatomical manifestations and detection methods].Der Pathologe · 2011Review
- Precision across the analytical measuring range of a quantitative real-time PCR assay for cytomegalovirus detection among three clinical laboratories.Journal of clinical microbiology · 2011Article
- Rapid quantitation of cytomegalovirus DNA in whole blood by a new molecular assay based on automated sample preparation and real-time PCR.Medical microbiology and immunology · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viralload (VL) assessment of cytomegalovirus (CMV) by real-time PCR is an important tool for diagnosing and monitoring CMV viremia in patients with compromised immune systems. We report results from a sample exchange organized by members of the Association for Molecular Pathology that compared PCR results from 23 laboratories; 22 such laboratories used a laboratory-developed real-time PCR assay and one laboratory used a competitive PCR assay. The samples sent to each laboratory were comprised of a dilution panel of CMV virion-derived reference materials that ranged from 0 to 500,000 copies/ml. Accuracy, linearity, and intralaboratory precision were established for the different laboratory-developed assays. Overall, PCR results were linear for each laboratory (R(2) > 0.97 in all but two). While 13 laboratories showed no significant quantitative assay bias, 10 laboratories reported VLs that were significantly different compared with expected values (bias range, -0.82 to 1.4 logs). The intralaboratory precision [mean coefficient of variance of 2% to 5% (log-scale)] suggested that changes in VLs of less than 3- to fivefold may not be significantly different. There was no significant association between laboratory-specific technical variables (PCR platform, calibrator, extraction method) and assay linearity or accuracy. These data suggested that, within each laboratory, relative VL values were linear, but additional method standardization and a CMV DNA reference standard are needed to allow laboratories to achieve comparable numeric results.
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