ArticleProceedings of the National Academy of Sciences of the United States of America2009
A bacterial type III effector family uses the papain-like hydrolytic activity to arrest the host cell cycle.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 69 citations in OpenAlex.
- Review
- Human Milk Oligosaccharides Inhibit Group B Streptococcal Growth by Binding PcsB, an Essential Cell Wall Separation Protein.JACS Au · 2026Article
- Article
- Hijacking the Ubl code: bacterial manipulation of ubiquitin-like proteins.Essays in biochemistry · 2025Review
- Bacterial small molecule metabolites implicated in gastrointestinal cancer development.Nature reviews. Microbiology · 2025Review
- Pathogenicity and virulence ofVirulence · 2022Review
- Cycle-Inhibiting Factor Is Associated withBiology · 2022Article
- Interesting Biochemistries in the Structure and Function of Bacterial Effectors.Frontiers in cellular and infection microbiology · 2021Review
- Bacterial virulence mediated by orthogonal post-translational modification.Nature chemical biology · 2020Review
- The differential strain virulence of the candidate toxins of3 Biotech · 2020Article
- Staying out or Going in? The Interplay between Type 3 and Type 5 Secretion Systems in Adhesion and Invasion of Enterobacterial Pathogens.International journal of molecular sciences · 2020Review
- Molecular Basis of Ubiquitination Catalyzed by the Bacterial Transglutaminase MavC.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Legionella effector MavC targets the Ube2N~Ub conjugate for noncanonical ubiquitination.Nature communications · 2020Article
- Legionella pneumophila regulates the activity of UBE2N by deamidase-mediated deubiquitination.The EMBO journal · 2020Article
- Divergence of Legionella Effectors Reversing Conventional and Unconventional Ubiquitination.Frontiers in cellular and infection microbiology · 2020Review
- Characterisation of cellular effects ofBiology open · 2018Article
- Sequence determinants of specific pattern-recognition of bacterial ligands by the NAIP-NLRC4 inflammasome.Cell discovery · 2018Article
- Host Cell S Phase RestrictsmBio · 2017Article
- Activation of MAPK/ERK signaling by Burkholderia pseudomallei cycle inhibiting factor (Cif).PloS one · 2017Article
- Clustered Intracellular Salmonella enterica Serovar Typhimurium Blocks Host Cell Cytokinesis.Infection and immunity · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pathogenic bacteria deliver effector proteins into host cells through the type III secretion apparatus to modulate the host function. We identify a family of proteins, homologous to the type III effector Cif from enteropathogenic Escherichia coli, in pathogens including Yersinia, Photorhabdus, and Burkholderia that contain functional type III secretion systems. Like Cif, this family of proteins is capable of arresting the host cell cycle at G(2)/M. Structure of one of the family members, Cif homolog in Burkholderia pseudomallei (CHBP), reveals a papain-like fold and a conserved Cys-His-Gln catalytic triad despite the lack of primary sequence identity. For CHBP and Cif, only the putative catalytic Cys is susceptible to covalent modification by E-64, a specific inhibitor of papain-like cysteine proteases. Unlike papain-like enzymes where the S2 site is the major determinant of cleavage-site specificity, CHBP has a characteristic negatively charged pocket occupying surface areas corresponding to the S1/S1' site in papain-like proteases. The negative charge is provided by a conserved aspartate, and the pocket best fits an arginine, as revealed by molecular docking analysis. Mutation analysis establishes the essential role of the catalytic triad and the negatively charged pocket in inducing cell cycle arrest in host cells. Our results demonstrate that bacterial pathogens have evolved a unique papain-like hydrolytic activity to block the normal host cell cycle progression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.