ArticleJournal of clinical microbiology2009
Evaluation of a nested-PCR-derived sequence-based typing method applied directly to respiratory samples from patients with Legionnaires' disease.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- MixedInfectious disease reports · 2026Article
- Preliminary report: The most considerable outbreak of Legionnaires' disease in France in the last two decades, Albertville, September 2025.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025Article
- A community Legionnaires' disease outbreak linked to a collective biomass condensing boiler, France, 2019.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025Article
- Cluster of Legionellosis Cases Associated with Manufacturing Process, South Carolina, USA, 2022.Emerging infectious diseases · 2025Article
- The Adequacy of Current Legionnaires' Disease Diagnostic Practices in Capturing the Epidemiology of Clinically RelevantPathogens (Basel, Switzerland) · 2024Article
- Characterisation ofMicroorganisms · 2024Article
- Culture-Free Phylogenetic Analysis of Legionella pneumophila Using Targeted CRISPR/Cas9 Next-Generation Sequencing.Microbiology spectrum · 2022Article
- A Community Outbreak of Legionnaires' Disease with Two Strains ofInternational journal of environmental research and public health · 2022Article
- Article
- Fatal Case of Nosocomial Legionella pneumophila Pneumonia, Spain, 2018.Emerging infectious diseases · 2019Article
- Legionella SBT applied directly to respiratory samples as a rapid molecular epidemiological tool.Scientific reports · 2019Article
- Comparative genome analysis reveals a complex population structure of Legionella pneumophila subspecies.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2018Article
- Genomic investigation of a suspected outbreak of Legionella pneumophila ST82 reveals undetected heterogeneity by the present gold-standard methods, Denmark, July to November 2014.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2017Article
- Energy Conservation and the Promotion of Legionella pneumophila Growth: The Probable Role of Heat Exchangers in a Nosocomial Outbreak.Infection control and hospital epidemiology · 2016Article
- Combined use of real-time PCR and nested sequence-based typing in survey of human Legionella infection.Epidemiology and infection · 2016Article
- Design and validation of a qPCR assay for accurate detection and initial serogrouping of Legionella pneumophila in clinical specimens by the ESCMID Study Group for Legionella Infections (ESGLI).European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Current and emerging Legionella diagnostics for laboratory and outbreak investigations.Clinical microbiology reviews · 2015Review
- Genomic Investigation of a Legionellosis Outbreak in a Persistently Colonized Hotel.Frontiers in microbiology · 2015Article
- Epidemiologic characteristics associated with ST23 clones compared to ST1 and ST47 clones of Legionnaires disease cases in France.New microbes and new infections · 2015Article
- Management of the 2012 Legionella crisis in Quebec City: need for a better communication between resources and knowledge transfer.Frontiers in microbiology · 2014Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequence-based typing (SBT) is a powerful method based on the sequencing of seven genes of Legionella pneumophila isolates. SBT performed directly on clinical samples has been used only in a limited number of cases. In our study, its efficiency was tested with 63 legionellosis respiratory samples. Sixty-three clinical samples, which included 23 samples from sporadic cases and 40 collected during four French outbreaks, confirmed by culture or urinary antigen testing and all positive by L. pneumophila quantitative PCR were subtyped by SBT according to the European Working Group for Legionella Infections standard scheme. Only 28.6% of the samples provided nucleotide sequences by SBT. Nested-PCR-based SBT (NPSBT) applied to the same respiratory samples was thus evaluated with new PCR primers surrounding the first set of primers used for the SBT. Sequencing results were obtained with 90.5% of the samples. Complete allelic profiles (seven genes sequenced) were obtained for 3.2% versus 53.9% of the samples by SBT and NPSBT, respectively. More importantly, of the 28 culture-negative samples, only 4 did not give any sequencing results. Taken together, NPSBT applied directly to clinical specimens significantly improved epidemiological typing compared to the initial SBT, in particular when no isolates are available.
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