ArticleClinical and vaccine immunology : CVI2009
Development of an immunochromatographic test for rapid serodiagnosis of human pythiosis.
Article in Clinical and vaccine immunology : CVI, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 68 citations in OpenAlex.
- Preliminary Validation of a Colorimetric Loop-Mediated Isothermal Amplification (c-LAMP) Assay for Detection ofJournal of fungi (Basel, Switzerland) · 2026Article
- Simplified Point-of-Care Testing for Human Pythiosis: Development of a Whole-Blood-Based Lateral Flow Immunoassay.Diagnostics (Basel, Switzerland) · 2026Article
- A rare case of pythium brain infection presenting as severe neurological deterioration in a pediatric patient: A case report and literature review.Radiology case reports · 2026Article
- Development of lateral flow immunochromatographic assay with Anti-Pythium insidiosum antibodies for point-of-care testing of vascular pythiosis.Scientific reports · 2025Article
- A colorimetric loop-mediated isothermal amplification assay (c-LAMP) for rapid detection ofHeliyon · 2024Article
- Article
- Infection with Pythium flevoense in a harbour porpoise (Phocoena phocoena) as a novel cause of dermatitis in marine mammals.Veterinary research · 2023Article
- Comparative Genomic Analysis Reveals Gene Content Diversity, Phylogenomic Contour, Putative Virulence Determinants, and Potential Diagnostic Markers withinJournal of fungi (Basel, Switzerland) · 2023Article
- Pythiosis presenting with chronic swelling and painful subcutaneous lesion at right deltoid.IDCases · 2023Article
- Nucleic Acid-Based Detection ofJournal of fungi (Basel, Switzerland) · 2022Review
- Selection of an Appropriate In Vitro Susceptibility Test for Assessing Anti-Journal of fungi (Basel, Switzerland) · 2022Article
- A Review: Antimicrobial Therapy for Human Pythiosis.Antibiotics (Basel, Switzerland) · 2022Review
- Global Distribution and Clinical Features of Pythiosis in Humans and Animals.Journal of fungi (Basel, Switzerland) · 2022Article
- History and Perspective of Immunotherapy for Pythiosis.Vaccines · 2021Review
- Immunological Cross-Reactivity of Proteins Extracted from the OomyceteJournal of fungi (Basel, Switzerland) · 2021Article
- Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis.Journal of fungi (Basel, Switzerland) · 2021Article
- Identification and Biotyping ofJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Review
- Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects.BMC research notes · 2020Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human pythiosis is an emerging and life-threatening infectious disease caused by the fungus-like organism Pythium insidiosum. High rates of morbidity and mortality for patients with pythiosis are exacerbated by the lack of early diagnosis and an effective treatment. Here, we developed and evaluated an immunochromatographic test (ICT) for the diagnosis of human pythiosis, in comparison to a standard serological test of immunodiffusion (ID). Culture filtrate antigen of P. insidiosum was used to detect human anti-P. insidiosum antibody. Sheep anti-human immunoglobulin G-colloidal gold conjugate was used to generate an ICT signal. Thirty-three sera from patients with vascular (n = 27), ocular (n = 4), and cutaneous (n = 2) pythiosis and 181 control sera from healthy blood donors (n = 100), as well as patients with a variety of infectious (n = 56) and noninfectious (n = 25) diseases, were included in the test evaluation. The turnaround time for generating a result by the ICT was less than 30 min, while that for ID was approximately 24 h. Based on the results for all sera of pythiosis patients and the control groups, the ICT showed 88% sensitivity and 100% specificity and ID showed 61% sensitivity and 100% specificity. By both tests, false-negative results for sera from all ocular pythiosis patients were obtained. In addition, the ID test yielded false-negative results for sera from eight patients with vascular pythiosis and one patient with cutaneous pythiosis. It was concluded that the ICT is a rapid, user-friendly, and reliable serological test for the early diagnosis of vascular and cutaneous pythiosis.
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