ArticleThe Plant cell2009
The Arabidopsis proteasome RPT5 subunits are essential for gametophyte development and show accession-dependent redundancy.
Article in The Plant cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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33 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- Role of the Genetic Background in Resistance to Plant Viruses.International journal of molecular sciences · 2018Pooled it
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- The Arabidopsis KASH protein SINE3 is involved in male and female gametogenesis.Plant reproduction · 2024Article
- The Identification and Gene Mapping of Spotted Leaf MutantInternational journal of molecular sciences · 2024Article
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- Identification of miRNA-eQTLs in maize mature leaf by GWAS.BMC genomics · 2020Article
- Abnormal leaf development of rpt5a mutant under zinc deficiency reveals important role of DNA damage alleviation for normal leaf development.Scientific reports · 2019Article
- Proteasomal degradation of BRAHMA promotes Boron tolerance in Arabidopsis.Nature communications · 2018Article
- iTRAQ-Based Proteomics Analyses of Sterile/Fertile Anthers from a Thermo-Sensitive Cytoplasmic Male-Sterile Wheat withInternational journal of molecular sciences · 2018Article
- Comparative proteomic study of Arabidopsis mutants mpk4 and mpk6.Scientific reports · 2016Article
- The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins.Plant molecular biology · 2016Article
- The Proteasome Stress Regulon Is Controlled by a Pair of NAC Transcription Factors in Arabidopsis.The Plant cell · 2016Article
- ahg12 is a dominant proteasome mutant that affects multiple regulatory systems for germination of Arabidopsis.Scientific reports · 2016Article
- Cyclic AMP deficiency negatively affects cell growth and enhances stress-related responses in tobacco Bright Yellow-2 cells.Plant molecular biology · 2016Article
- Natural alleles of a proteasome α2 subunit gene contribute to thermotolerance and adaptation of African rice.Nature genetics · 2015Article
- SUMO E3 ligase AtMMS21 is required for normal meiosis and gametophyte development in Arabidopsis.BMC plant biology · 2014Article
- Functional redundancy and/or ongoing pseudogenization among F-box protein genes expressed in Arabidopsis male gametophyte.Plant reproduction · 2014Article
- Suppression of the auxin response pathway enhances susceptibility to Phytophthora cinnamomi while phosphite-mediated resistance stimulates the auxin signalling pathway.BMC plant biology · 2014Article
- Proteomic analysis of MG132-treated germinating pollen reveals expression signatures associated with proteasome inhibition.PloS one · 2014Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the role of the ubiquitin proteasome system (UPS), which allows proteins to be selectively degraded, during gametophyte development in Arabidopsis thaliana. Three mutant alleles altering the UPS were isolated in the Wassilewskija (Ws) accession: they affect the Regulatory Particle 5a (RPT5a) gene, which (along with RPT5b) encodes one of the six AAA-ATPases of the proteasome regulatory particle. In the heterozygous state, all three mutant alleles displayed 50% pollen lethality, suggesting that RPT5a is essential for male gametophyte development. However, a fourth mutant in the Columbia (Col) accession did not display such a phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the RPT5b Ws allele does not. Double rpt5a rpt5b mutants showed a complete male and female gametophyte lethal phenotype in a Col background, indicating that RPT5 subunits are essential for both gametophytic phases. Mitotic divisions were affected in double mutant gametophytes correlating with an absence of the proteasome-dependent cyclinA3 degradation. Finally, we show that RPT5b expression is highly increased when proteasome functioning is defective, allowing complementation of the rpt5a mutation. In conclusion, RPT5 subunits are not only essential for both male and female gametophyte development but also display accession-dependent redundancy and are crucial in cell cycle progression.
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