ArticleInfection and immunity2009
First Streptococcus pyogenes signature-tagged mutagenesis screen identifies novel virulence determinants.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 49 citations in OpenAlex.
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- A teichoic acid-like wall modification associated with immune suppression is socially regulated inbioRxiv : the preprint server for biology · 2026Article
- Zebrafish (International journal of molecular sciences · 2024Review
- Streptococcal peptides and their roles in host-microbe interactions.Frontiers in cellular and infection microbiology · 2023Review
- Quorum Sensing Regulation of a Major Facilitator Superfamily Transporter Affects Multiple Streptococcal Virulence Factors.Journal of bacteriology · 2022Article
- AmBio · 2021Article
- A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans.Journal of microbiology (Seoul, Korea) · 2020Article
- Streptococcus pyogenes genes that promote pharyngitis in primates.JCI insight · 2020Article
- Article
- The Diverse Roles of Phagocytes During Bacterial and Fungal Infections and Sterile Inflammation: Lessons From Zebrafish.Frontiers in immunology · 2020Review
- Genetics of Group A Streptococci.Microbiology spectrum · 2019Review
- Gene fitness landscape of group A streptococcus during necrotizing myositis.The Journal of clinical investigation · 2019Article
- Emergence of a Streptococcus dysgalactiae subspecies equisimilis stG62647-lineage associated with severe clinical manifestations.Scientific reports · 2017Article
- High-Throughput Genetic Screen Reveals that Early Attachment and Biofilm Formation Are Necessary for Full Pyoverdine Production byFrontiers in microbiology · 2017Article
- Identification of a two-component Class IIb bacteriocin in Streptococcus pyogenes by recombinase-based in vivo expression technology.Scientific reports · 2016Article
- TheFrontiers in microbiology · 2016Article
- Neutrophils in host defense: new insights from zebrafish.Journal of leukocyte biology · 2015Review
- Induction of a quorum sensing pathway by environmental signals enhances group A streptococcal resistance to lysozyme.Molecular microbiology · 2015Article
- Copper Tolerance and Characterization of a Copper-Responsive Operon, copYAZ, in an M1T1 Clinical Strain of Streptococcus pyogenes.Journal of bacteriology · 2015Article
- Essential Genes in the Core Genome of the Human Pathogen Streptococcus pyogenes.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The virulence of bacterial pathogens is a complex process that requires the dynamic expression of many genes for the pathogens to invade and circumvent host defenses, as well as to proliferate in vivo. In this study, we employed a large-scale screen, signature-tagged mutagenesis (STM), to identify Streptococcus pyogenes virulence genes important for pathogenesis within the host. Approximately 1,200 STM mutants were created and screened using the zebrafish infectious disease model. The transposon insertion site was identified for 29 of the 150 mutants that were considered attenuated for virulence. Previously reported streptococcal virulence genes, such as mga, hasA, amrA, smeZ, and two genes in the sil locus, were identified, confirming the utility of the model for revealing genes important for virulence. Multiple genes not previously implicated in virulence were also identified, including genes encoding putative transporters, hypothetical cytosolic proteins, and macrolide efflux pumps. The STM mutant strains display various levels of attenuation, and multiple separate insertions were identified in either the same gene or the same locus, suggesting that these factors are important for this type of acute, invasive infection. We further examined two such genes, silB and silC of a putative quorum-sensing regulon, and determined that they are significant virulence factors in our model of necrotizing fasciitis. sil locus promoter expression was examined under various in vitro conditions, as well as in zebrafish tissues, and was found to be differentially induced. This study was a unique investigation of S. pyogenes factors required for successful invasive infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.