ArticleMolecular and cellular biology2009
Transforming growth factor beta depletion is the primary determinant of Smad signaling kinetics.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 66 citations in OpenAlex.
- FKBP12 determines ligand-specific SMAD signaling in individual living endothelial cells.iScience · 2026Article
- Deletion of Megalin in Kidney Tubular Epithelium Up-Regulates TGFβ1 Signaling, Aggravates Ischemia/Reperfusion Kidney Injury, and Accelerates the Progression to Chronic Kidney Disease.The American journal of pathology · 2026Article
- Transcriptional regulators ensuring specific gene expression and decision-making at high TGFβ doses.Life science alliance · 2025Article
- Data-based stochastic modeling reveals sources of activity bursts in single-cell TGF-β signaling.PLoS computational biology · 2022Article
- Emerging Roles of Disabled Homolog 2 (DAB2) in Immune Regulation.Frontiers in immunology · 2020Review
- SMAD3 directly regulates cell cycle genes to maintain arrest in granulosa cells of mouse primordial follicles.Scientific reports · 2019Article
- Engineering Approaches to Study Cellular Decision Making.Annual review of biomedical engineering · 2018Review
- Spatiotemporal Control of TGF-β Signaling with Light.ACS synthetic biology · 2018Article
- Cell-specific responses to the cytokine TGFβ are determined by variability in protein levels.Molecular systems biology · 2018Article
- Induction of senescence in primary glioblastoma cells by serum and TGFβ.Scientific reports · 2017Article
- Mathematical model of TGF-βsignalling: feedback coupling is consistent with signal switching.BMC systems biology · 2017Article
- Article
- Immobilized epidermal growth factor stimulates persistent, directed keratinocyte migration via activation of PLCγ1.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Measuring TGF-β Ligand Dynamics in Culture Medium.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Studying Cellular Signal Transduction with OMIC Technologies.Journal of molecular biology · 2015Review
- Covalently tethered TGF-β1 with encapsulated chondrocytes in a PEG hydrogel system enhances extracellular matrix production.Journal of biomedical materials research. Part A · 2014Article
- Analysis of the quantitative balance between insulin-like growth factor (IGF)-1 ligand, receptor, and binding protein levels to predict cell sensitivity and therapeutic efficacy.BMC systems biology · 2014Article
- The self-limiting dynamics of TGF-β signaling in silico and in vitro, with negative feedback through PPM1A upregulation.PLoS computational biology · 2014Article
- Sustained delivery of bioactive TGF-β1 from self-assembling peptide hydrogels induces chondrogenesis of encapsulated bone marrow stromal cells.Journal of biomedical materials research. Part A · 2014Article
- Loss of Dab2 expression in breast cancer cells impairs their ability to deplete TGF-β and induce Tregs development via TGF-β.PloS one · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
A cell's decision to growth arrest, apoptose, or differentiate in response to transforming growth factor beta (TGF-beta) superfamily ligands depends on the ligand concentration. How cells sense the concentration of extracellular bioavailable TGF-beta remains poorly understood. We therefore undertook a systematic quantitative analysis of how TGF-beta ligand concentration is transduced into downstream phospho-Smad2 kinetics, and we found that the rate of TGF-beta ligand depletion is the principal determinant of Smad signal duration. TGF-beta depletion is caused by two mechanisms: (i) cellular uptake of TGF-beta by a TGF-beta type II receptor-dependent mechanism and (ii) reversible binding of TGF-beta to the cell surface. Our results indicate that cells sense TGF-beta dose by depleting TGF-beta via constitutive TGF-beta type II receptor trafficking processes. Our results also have implications for the role of the TGF-beta type II receptor in disease, as tumor cells harboring TGF-beta type II receptor mutations exhibit impaired TGF-beta depletion, which may contribute to the overproduction of TGF-beta and a consequently poor prognosis in cancer.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.