ArticleNucleic acids research2009
Targeting individual subunits of the FokI restriction endonuclease to specific DNA strands.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- Fusions of Catalytically Inactive RusA to FokI Nuclease Coupled with PNA Enable Programmable Site-Specific Double-Stranded DNA Breaks.ACS omega · 2025Article
- Strand-preferred base editing of organellar and nuclear genomes using CyDENT.Nature biotechnology · 2024Article
- Structures, activity and mechanism of the Type IIS restriction endonuclease PaqCI.Nucleic acids research · 2023Article
- Engineering the plastid and mitochondrial genomes of flowering plants.Nature plants · 2022Review
- Targeted introduction of heritable point mutations into the plant mitochondrial genome.Nature plants · 2022Article
- TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Modification-dependent restriction endonuclease, MspJI, flips 5-methylcytosine out of the DNA helix.Nucleic acids research · 2014Article
- Type II restriction endonucleases--a historical perspective and more.Nucleic acids research · 2014Review
- Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.Nucleic acids research · 2014Article
- Terminal protection of small molecule-linked DNA for small molecule-protein interaction assays.International journal of molecular sciences · 2014Review
- Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats.Nucleic acids research · 2013Article
- Organization of the BcgI restriction-modification protein for the cleavage of eight phosphodiester bonds in DNA.Nucleic acids research · 2013Article
- Natural zinc ribbon HNH endonucleases and engineered zinc finger nicking endonuclease.Nucleic acids research · 2013Article
- Zinc-finger nickase-mediated insertion of the lysostaphin gene into the beta-casein locus in cloned cows.Nature communications · 2013Article
- Structure and cleavage activity of the tetrameric MspJI DNA modification-dependent restriction endonuclease.Nucleic acids research · 2012Article
- Precision genome engineering with programmable DNA-nicking enzymes.Genome research · 2012Article
- Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects.Nucleic acids research · 2012Article
- Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme.Genome research · 2012Article
- DNA looping by FokI: the impact of twisting and bending rigidity on protein-induced looping dynamics.Nucleic acids research · 2012Article
- DNA looping by FokI: the impact of synapse geometry on loop topology at varied site orientations.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Many restriction endonucleases are dimers that act symmetrically at palindromic DNA sequences, with each active site cutting one strand. In contrast, FokI acts asymmetrically at a non-palindromic sequence, cutting 'top' and 'bottom' strands 9 and 13 nucleotides downstream of the site. FokI is a monomeric protein with one active site and a single monomer covers the entire recognition sequence. To cut both strands, the monomer at the site recruits a second monomer from solution, but it is not yet known which DNA strand is cut by the monomer bound to the site and which by the recruited monomer. In this work, mutants of FokI were used to show that the monomer bound to the site made the distal cut in the bottom strand, whilst the recruited monomer made in parallel the proximal cut in the top strand. Procedures were also established to direct FokI activity, either preferentially to the bottom strand or exclusively to the top strand. The latter extends the range of enzymes for nicking specified strands at specific sequences, and may facilitate further applications of FokI in gene targeting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.