ArticleNucleic acids research2009
A genetic screen for components of the mammalian RNA interference pathway in Bloom-deficient mouse embryonic stem cells.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 12 citations in OpenAlex.
- Screening for stress-resistance mutations in the mouse.Frontiers in genetics · 2014Article
- Mobile elements in the human genome: implications for disease.Genome medicine · 2012Article
- Isolation of homozygous mutant mouse embryonic stem cells using a dual selection system.Nucleic acids research · 2012Article
- Mammalian hyperplastic discs homolog EDD regulates miRNA-mediated gene silencing.Molecular cell · 2011Article
- Derivation and characterization of haploid embryonic stem cell cultures in medaka fish.Nature protocols · 2010Article
- Gene trap mutagenesis in the mouse.Methods in enzymology · 2010Article
- PiggyBac transgenic strategies in the developing chicken spinal cord.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Genetic screens performed in model organisms have helped identify key components of the RNA interference (RNAi) pathway. Recessive genetic screens have recently become feasible through the use of mouse embryonic stem (ES) cells that are Bloom's syndrome protein (Blm) deficient. Here, we developed and performed a recessive genetic screen to identify components of the mammalian RNAi pathway in Blm-deficient ES cells. Genome-wide mutagenesis using a retroviral gene trap strategy resulted in the isolation of putative homozygous RNAi mutant cells. Candidate clones were confirmed by an independent RNAi-based reporter assay and the causative gene trap integration site was identified using molecular techniques. Our screen identified multiple mutant cell lines of Argonaute 2 (Ago2), a known essential component of the RNAi pathway. This result demonstrates that true RNAi components can be isolated by this screening strategy. Furthermore, Ago2 homozygous mutant ES cells provide a null genetic background to perform mutational analyses of the Ago2 protein. Using genetic rescue, we resolve an important controversy regarding the role of two phenylalanine residues in Ago2 activity.
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Registered trials
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