ArticleBiochemical and biophysical research communications2009
Regulation of ROCKII by localization to membrane compartments and binding to DynaminI.
Article in Biochemical and biophysical research communications, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- Polarized E-cadherin endocytosis directs actomyosin remodeling during embryonic wound repair.The Journal of cell biology · 2015Article
- Regulation of ROCK activity in cancer.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Review
- Structure of a highly conserved domain of Rock1 required for Shroom-mediated regulation of cell morphology.PloS one · 2013Article
- Rho-associated kinases in tumorigenesis: re-considering ROCK inhibition for cancer therapy.EMBO reports · 2012Review
- Novel mechanism for negatively regulating Rho-kinase (ROCK) signaling through Coronin1B protein in neuregulin 1 (NRG-1)-induced tumor cell motility.The Journal of biological chemistry · 2012Article
- Regulation of ROCKII membrane localization through its C-terminus.Experimental cell research · 2011Article
- Shroom2 regulates contractility to control endothelial morphogenesis.Molecular biology of the cell · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
ROCKII kinase activity is known to be regulated by Rho GTPase binding; however, the context-specific regulation of ROCKII is not clearly understood. We pursued the C-terminal PH domain as a candidate domain for regulating ROCKII function. A proteomics-based screen identified potential ROCKII signaling partners, a large number of which were associated with membrane dynamics. We used subcellular fractionation to demonstrate that ROCKII is localized to both the plasma membrane and internal endosomal membrane fractions, and then used microscopy to show that the C-terminal PH domain can localize to internal or peripheral membrane compartments, depending on the cellular context. Co-immunoprecipitation demonstrated that Dynamin1 is a novel ROCKII binding partner. Furthermore, blocking Dynamin function with a dominant negative mutant mimicked the effect of inhibiting ROCK activity on the actin cytoskeleton. Our data suggest that ROCKII is regulated by localization to specific membrane compartments and its novel binding partner, Dynamin1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.