ArticleBMC microbiology2009
Short hairpin RNA-mediated knockdown of protein expression in Entamoeba histolytica.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Work with me here: variations in genome content and emerging genetic tools in Entamoeba histolytica.Trends in parasitology · 2025Review
- Are Metabolites From the Gut Microbiota Capable of Regulating Epigenetic Mechanisms in the Human ParasiteFrontiers in cell and developmental biology · 2022Review
- Growth and Genetic Manipulation of Entamoeba histolytica.Current protocols · 2022Article
- Establishment of quantitative RNAi-based forward genetics in Entamoeba histolytica and identification of genes required for growth.PLoS pathogens · 2021Article
- Developmental Sensitivity in Schistosoma mansoni to Puromycin To Establish Drug Selection of Transgenic Schistosomes.Antimicrobial agents and chemotherapy · 2018Article
- Comparison of hemolytic activity of the intermediate subunit of Entamoeba histolytica and Entamoeba dispar lectins.PloS one · 2017Article
- Development of RNA Interference Trigger-Mediated Gene Silencing in Entamoeba invadens.Infection and immunity · 2016Article
- Destabilization domain approach adapted for regulated protein expression in the protozoan parasite Entamoeba histolytica.International journal for parasitology · 2014Article
- Crystal structure and putative substrate identification for the Entamoeba histolytica low molecular weight tyrosine phosphatase.Molecular and biochemical parasitology · 2014Article
- Robust gene silencing mediated by antisense small RNAs in the pathogenic protist Entamoeba histolytica.Nucleic acids research · 2013Article
- Evidence for a bacterial lipopolysaccharide-recognizing G-protein-coupled receptor in the bacterial engulfment by Entamoeba histolytica.Eukaryotic cell · 2013Article
- Effect of the silencing of the Ehcp112 gene on the in vitro virulence of Entamoeba histolytica.Parasites & vectors · 2013Article
- Small RNA pyrosequencing in the protozoan parasite Entamoeba histolytica reveals strain-specific small RNAs that target virulence genes.BMC genomics · 2013Article
- Entamoeba histolytica: a snapshot of current research and methods for genetic analysis.Current opinion in microbiology · 2012Review
- A Multilocus Sequence Typing System (MLST) reveals a high level of diversity and a genetic component to Entamoeba histolytica virulence.BMC microbiology · 2012Article
- Human U6 promoter drives stronger shRNA activity than its schistosome orthologue in Schistosoma mansoni and human fibrosarcoma cells.Transgenic research · 2012Article
- Entamoeba histolytica cell surface calreticulin binds human c1q and functions in amebic phagocytosis of host cells.Infection and immunity · 2012Article
- Genetic manipulation of schistosomes--progress with integration competent vectors.Parasitology · 2012Review
- Schistosoma mansoni U6 gene promoter-driven short hairpin RNA induces RNA interference in human fibrosarcoma cells and schistosomules.International journal for parasitology · 2011Article
- Tissue destruction and invasion by Entamoeba histolytica.Trends in parasitology · 2011Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundEntamoeba histolytica is an intestinal protozoan parasite of humans. The genome has been sequenced, but the study of individual gene products has been hampered by the lack of the ability to generate gene knockouts. We chose to test the use of RNA interference to knock down gene expression in Entamoeba histolytica.
resultsAn episomal vector-based system, using the E. histolytica U6 promoter to drive expression of 29-basepair short hairpin RNAs, was developed to target protein-encoding genes in E. histolytica. The short hairpin RNAs successfully knocked down protein levels of all three unrelated genes tested with this system: Igl, the intermediate subunit of the galactose- and N-acetyl-D-galactosamine-inhibitable lectin; the transcription factor URE3-BP; and the membrane binding protein EhC2A. Igl levels were reduced by 72%, URE3-BP by 89%, and EhC2A by 97%.
conclusionUse of the U6 promoter to drive expression of 29-basepair short hairpin RNAs is effective at knocking down protein expression for unrelated genes in Entamoeba histolytica, providing a useful tool for the study of this parasite.
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