ArticleThe Journal of biological chemistry2009
Shift from apoptotic to necrotic cell death during human papillomavirus-induced transformation of keratinocytes.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Human Papillomavirus Drives Tumor Development Throughout the Head and Neck: Improved Prognosis Is Associated With an Immune Response Largely Restricted to the Oropharynx.Journal of clinical oncology : official journal of the American Society of Clinical Oncology · 2016Pooled it
- Article
- Computational surprisal analysis speeds-up genomic characterization of cancer processes.PloS one · 2014Article
- Article
- Article
- On a fundamental structure of gene networks in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Effects of human papillomavirus (HPV) type 16 oncoproteins on the expression of involucrin in human keratinocytes.Virology journal · 2012Article
- Viral perturbations of host networks reflect disease etiology.PLoS computational biology · 2012Article
- cIAPs block Ripoptosome formation, a RIP1/caspase-8 containing intracellular cell death complex differentially regulated by cFLIP isoforms.Molecular cell · 2011Article
- Convergence of logic of cellular regulation in different premalignant cells by an information theoretic approach.BMC systems biology · 2011Article
- Status of p53 in human cancer cells does not predict efficacy of CHK1 kinase inhibitors combined with chemotherapeutic agents.Oncogene · 2010Article
- Information-theoretic analysis of phenotype changes in early stages of carcinogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- A color discriminating broad range cell staining technology for early detection of cell transformation.Journal of carcinogenesis · 2009Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oncogenic transformation is a complex, multistep process, which goes through several stages before complete malignant transformation occurs. To identify early processes in carcinogenesis, we used an in vitro model, based on the initiating event in cervical cancer, papillomavirus transformation of keratinocytes. We compared gene expression in primary keratinocytes (K) and papillomavirus-transformed keratinocytes from early (E) and late (L) passages and from benzo[a]pyrene-treated L cells (BP). The transformed cells exhibit similar transcriptional changes to clinical cervical carcinoma. The number of transcripts expressed progressively decreased during the evolution from K to BP cells. Bioinformatic analysis, validated by detailed biochemical analysis, revealed substantial contraction of both pro- and antiapoptotic networks during transformation. Nonetheless, L and BP cells were not resistant to apoptotic stimuli. At doses of cisplatin that led to 30-60% apoptosis of K and E cells, transformed L and BP cells underwent 80% necrotic cell death, which became the default response to genotoxic stress. Moreover, appreciable necrotic fractions were observed in the cervical carcinoma cell line, HeLa, in response to comparable doses of cisplatin. The shrinkage of biochemical networks, including the apoptotic network, may allow a cancer cell to economize on energy usage to facilitate enhanced proliferation but leaves it vulnerable to stress. This study supports the hypothesis that the process of cancer transformation may be accompanied by a shift from apoptosis to necrosis.
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