ArticleThe Journal of physiology2009
Interaction between the second messengers cAMP and Ca2+ in mouse presynaptic taste cells.
Article in The Journal of physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 43 citations in OpenAlex.
- Exogenous oral application of PYY and exendin-4 impacts upon taste-related behavior and taste perception in wild-type mice.Neuropharmacology · 2025Article
- Preferential allosteric modulation of Otop1 channels by small molecule compounds.Communications biology · 2025Article
- Intracellular cAMP signaling-induced CaPflugers Archiv : European journal of physiology · 2025Article
- The Expression of Cannabinoid and Cannabinoid-Related Receptors on the Gustatory Cells of the Piglet Tongue.Molecules (Basel, Switzerland) · 2024Article
- Defining the role of TRPM4 in broadly responsive taste receptor cells.Frontiers in cellular neuroscience · 2023Article
- Taste Receptor Signaling.Handbook of experimental pharmacology · 2022Article
- Mechanisms for the Sour Taste.Handbook of experimental pharmacology · 2022Article
- Cross-Talk Between the Adenylyl Cyclase/cAMP Pathway and CaReviews of physiology, biochemistry and pharmacology · 2021Review
- GAD65Cre Drives Reporter Expression in Multiple Taste Cell Types.Chemical senses · 2021Article
- Sensing Senses: Optical Biosensors to Study Gustation.Sensors (Basel, Switzerland) · 2020Review
- Spilanthol Enhances Sensitivity to Sodium in Mouse Taste Bud Cells.Chemical senses · 2019Article
- Chemical synapses without synaptic vesicles: Purinergic neurotransmission through a CALHM1 channel-mitochondrial signaling complex.Science signaling · 2018Article
- Type III Cells in Anterior Taste Fields Are More Immunohistochemically Diverse Than Those of Posterior Taste Fields in Mice.Chemical senses · 2017Article
- Whole transcriptome profiling of taste bud cells.Scientific reports · 2017Article
- Amiloride-Insensitive Salt Taste Is Mediated by Two Populations of Type III Taste Cells with Distinct Transduction Mechanisms.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Calcium signaling in taste cells.Biochimica et biophysica acta · 2015Review
- Angiotensin II modulates salty and sweet taste sensitivities.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Ryanodine Receptors Selectively Interact with L Type Calcium Channels in Mouse Taste Cells.PloS one · 2013Article
- Changes in taste receptor cell [Ca2+]i modulate chorda tympani responses to salty and sour taste stimuli.Journal of neurophysiology · 2012Article
- The cellular building blocks of breathing.Comprehensive Physiology · 2012Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The second messenger, 3',5'-cyclic adenosine monophosphate (cAMP), is known to be modulated in taste buds following exposure to gustatory and other stimuli. Which taste cell type(s) (Type I/glial-like cells, Type II/receptor cells, or Type III/presynaptic cells) undergo taste-evoked changes of cAMP and what the functional consequences of such changes are remain unknown. Using Fura-2 imaging of isolated mouse vallate taste cells, we explored how elevating cAMP alters Ca(2+) levels in identified taste cells. Stimulating taste buds with forskolin (Fsk; 1 microm) + isobutylmethylxanthine (IBMX; 100 microm), which elevates cellular cAMP, triggered Ca(2+) transients in 38% of presynaptic cells (n = 128). We used transgenic GAD-GFP mice to show that cAMP-triggered Ca(2+) responses occur only in the subset of presynaptic cells that lack glutamic acid decarboxylase 67 (GAD). We never observed cAMP-stimulated responses in receptor cells, glial-like cells or GAD-expressing presynaptic cells. The response to cAMP was blocked by the protein kinase A inhibitor H89 and by removing extracellular Ca(2+). Thus, the response to elevated cAMP is a PKA-dependent influx of Ca(2+). This Ca(2+) influx was blocked by nifedipine (an inhibitor of L-type voltage-gated Ca(2+) channels) but was unperturbed by omega-agatoxin IVA and omega-conotoxin GVIA (P/Q-type and N-type channel inhibitors, respectively). Single-cell RT-PCR on functionally identified presynaptic cells from GAD-GFP mice confirmed the pharmacological analyses: Ca(v)1.2 (an L-type subunit) is expressed in cells that display cAMP-triggered Ca(2+) influx, while Ca(v)2.1 (a P/Q subunit) is expressed in all presynaptic cells, and underlies depolarization-triggered Ca(2+) influx. Collectively, these data demonstrate cross-talk between cAMP and Ca(2+) signalling in a subclass of taste cells that form synapses with gustatory fibres and may integrate tastant-evoked signals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.